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The current study presents the development process and initial validation of a measure designed for assessing psychological needs satisfaction in a secondary school physical education context (Psychological Needs Satisfaction Scale in Physical Education, PNSSPE). Junior secondary school (grades 7 to 9) students (N = 1,258) were invited to participate in three studies. In Study 1, item generation (34 items) and initial content validity of the PNSSPE were achieved. In Study 2, the factorial structure of the PNSSPE was tested using exploratory and confirmatory factor analyses. Internal consistency reliabilities of the subscales were also examined. In Study 3, the reliability and validity of the scores derived from the PNSSPE were further examined in an independent sample. Overall, the PNSSPE demonstrated good content, factorial, discriminant, and nomological validities. It also demonstrated acceptable internal consistency and test–retest reliability. The measurement model was proven invariant across gender and samples. Psychometric evidence from a series of studies suggests that the PNSSPE could be used as a reliable and valid measure to assess Hong Kong secondary school students’ satisfaction of psychological needs in physical education. [ABSTRACT FROM AUTHOR]