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Your search keyword '"Weiss, Susan"' showing total 13 results
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13 results on '"Weiss, Susan"'

1. Comparison of SARS-CoV-2 variants of concern in primary human nasal cultures demonstrates Delta as most cytopathic and Omicron as fastest replicating

Author

Tanneti, Nikhila S., primary, Patel, Anant K., additional, Tan, Li Hui, additional, Marques, Andrew D., additional, Perera, Ranawaka A. P. M., additional, Sherrill-Mix, Scott, additional, Kelly, Brendan J., additional, Renner, David M., additional, Collman, Ronald G., additional, Rodino, Kyle, additional, Lee, Carole, additional, Bushman, Frederic D., additional, Cohen, Noam A., additional, and Weiss, Susan R., additional

Published
2024
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2. Outcomes of Convalescent Plasma with Defined High versus Lower Neutralizing Antibody Titers against SARS-CoV-2 among Hospitalized Patients: CoronaVirus Inactivating Plasma (CoVIP) Study

Author

Bartelt, Luther A., primary, Markmann, Alena J., additional, Nelson, Bridget, additional, Keys, Jessica, additional, Root, Heather, additional, Henderson, Heather I., additional, Kuruc, JoAnn, additional, Baker, Caroline, additional, Bhowmik, D. Ryan, additional, Hou, Yixuan J., additional, Premkumar, Lakshmanane, additional, Cornaby, Caleb, additional, Schmitz, John L., additional, Weiss, Susan, additional, Park, Yara, additional, Baric, Ralph, additional, de Silva, Aravinda M., additional, Lachiewicz, Anne, additional, Napravnik, Sonia, additional, van Duin, David, additional, and Margolis, David M., additional

Published
2022
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3. SARS-CoV-2 Diverges from Other Betacoronaviruses in Only Partially Activating the IRE1α/XBP1 Endoplasmic Reticulum Stress Pathway in Human Lung-Derived Cells

Author

Nguyen, Long C., primary, Renner, David M., additional, Silva, Diane, additional, Yang, Dongbo, additional, Parenti, Nicholas A., additional, Medina, Kaeri M., additional, Nicolaescu, Vlad, additional, Gula, Haley, additional, Drayman, Nir, additional, Valdespino, Andrea, additional, Mohamed, Adil, additional, Dann, Christopher, additional, Wannemo, Kristin, additional, Robinson-Mailman, Lydia, additional, Gonzalez, Alan, additional, Stock, Letícia, additional, Cao, Mengrui, additional, Qiao, Zeyu, additional, Moellering, Raymond E., additional, Tay, Savas, additional, Randall, Glenn, additional, Beers, Michael F., additional, Rosner, Marsha Rich, additional, Oakes, Scott A., additional, and Weiss, Susan R., additional

Published
2022
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4. Subcellular Detection of SARS-CoV-2 RNA in Human Tissue Reveals Distinct Localization in Alveolar Type 2 Pneumocytes and Alveolar Macrophages

Author

Acheampong, Kofi K., primary, Schaff, Dylan L., additional, Emert, Benjamin L., additional, Lake, Jonathan, additional, Reffsin, Sam, additional, Shea, Emily K., additional, Comar, Courtney E., additional, Litzky, Leslie A., additional, Khurram, Nigar A., additional, Linn, Rebecca L., additional, Feldman, Michael, additional, Weiss, Susan R., additional, Montone, Kathleen T., additional, Cherry, Sara, additional, and Shaffer, Sydney M., additional

Published
2022
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7. SARS-CoV-2 Genomic Variation in Space and Time in Hospitalized Patients in Philadelphia

Author

Everett, John, primary, Hokama, Pascha, additional, Roche, Aoife M., additional, Reddy, Shantan, additional, Hwang, Young, additional, Kessler, Lyanna, additional, Glascock, Abigail, additional, Li, Yize, additional, Whelan, Jillian N., additional, Weiss, Susan R., additional, Sherrill-Mix, Scott, additional, McCormick, Kevin, additional, Whiteside, Samantha A., additional, Graham-Wooten, Jevon, additional, Khatib, Layla A., additional, Fitzgerald, Ayannah S., additional, Collman, Ronald G., additional, and Bushman, Frederic, additional

Published
2021
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13. Subcellular Detection of SARS-CoV-2 RNA in Human Tissue Reveals Distinct Localization in Alveolar Type 2 Pneumocytes and Alveolar Macrophages.

Author

Acheampong KK, Schaff DL, Emert BL, Lake J, Reffsin S, Shea EK, Comar CE, Litzky LA, Khurram NA, Linn RL, Feldman M, Weiss SR, Montone KT, Cherry S, and Shaffer SM

Subjects
Humans, Macrophages, Alveolar, SARS-CoV-2, RNA, Viral, In Situ Hybridization, Fluorescence, Lung pathology, Alveolar Epithelial Cells, COVID-19
Abstract

The widespread coronavirus disease 2019 (COVID-19) is caused by infection with the novel coronavirus SARS-CoV-2. Currently, we have limited understanding of which cells become infected with SARS-CoV-2 in human tissues and where viral RNA localizes on the subcellular level. Here, we present a platform for preparing autopsy tissue for visualizing SARS-CoV-2 RNA using RNA fluorescence in situ hybridization (FISH) with amplification by hybridization chain reaction. We developed probe sets that target different regions of SARS-CoV-2 (including ORF1a and N), as well as probe sets that specifically target SARS-CoV-2 subgenomic mRNAs. We validated these probe sets in cell culture and tissues (lung, lymph node, and placenta) from infected patients. Using this technology, we observe distinct subcellular localization patterns of the ORF1a and N regions. In human lung tissue, we performed multiplexed RNA FISH HCR for SARS-CoV-2 and cell-type-specific marker genes. We found viral RNA in cells containing the alveolar type 2 (AT2) cell marker gene ( SFTPC ) and the alveolar macrophage marker gene ( MARCO ) but did not identify viral RNA in cells containing the alveolar type 1 (AT1) cell marker gene ( AGER ). Moreover, we observed distinct subcellular localization patterns of viral RNA in AT2 cells and alveolar macrophages. In sum, we demonstrate the use of RNA FISH HCR for visualizing different RNA species from SARS-CoV-2 in cell lines and FFPE (formalin fixation and paraffin embedding) autopsy specimens. We anticipate that this platform could be broadly useful for studying SARS-CoV-2 pathology in tissues, as well as extended for other applications, including investigating the viral life cycle, viral diagnostics, and drug screening. IMPORTANCE Here, we developed an in situ RNA detection assay for RNA generated by the SARS-CoV-2 virus. We found viral RNA in lung, lymph node, and placenta samples from pathology specimens from COVID patients. Using high-magnification microscopy, we can visualize the subcellular distribution of these RNA in single cells.

Published
2021
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