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The performance of the QuEChERS extraction followed by the LC-MS/MS analytical method was evaluated in terms of Alternaria mycotoxins recovery from the wheat. The alternariol (AOH), tentoxin (TEN) and alternariol monomethyl ether (AME) were analyzed using the ESI+ (electrospray positive ionization) by multiple reactions monitoring mode (MRM). In order to determine the recovery, the blank wheat samples were spiked at two spiking levels (0.01 and 0.1 mg/kg) in six replicates. The obtained average recoveries and precisions (expressed as the RSDr, %) were as follows: 107.6% (RSDr of 6.84%) for alternariol, 108.0% (RSDr of 6.78%) for tentoxin and 110.1% (RSDr of 6.50%) for alternariol monomethyl ether. The results of this study were in accordance with the Commission Decision 2002/657/EC and Commission Regulation (EC) No 401/2006. [ABSTRACT FROM AUTHOR]

Copyright of Matica Srpska Journal for Natural Sciences is the property of Matica Srpska, Department of Natural Studies and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The analysis of citrinin is challenging because it needs to be detected in low concentrations in complex sample matrices. Before citrinin quantification, the data acquisition of LC-MS/MS must be performed, which includes the determination of ion monitoring reaction (SRM), finding fragmentation energies (Frag.) and collision cell energies (CE) for which the response of citrinin will be the highest for the given conditions. The best response of citrinin is obtained for Frag. of 66 V and CE of 17 and 29 V. [ABSTRACT FROM AUTHOR]

Copyright of Matica Srpska Journal for Natural Sciences is the property of Matica Srpska, Department of Natural Studies and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Matica Srpska Journal for Natural Sciences is the property of Matica Srpska, Department of Natural Studies and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

A reliable and easy method has been developed for the determination of aflatoxins B1 and G2 in maize samples. High performance liquid chromatography coupled with FLD (HPLC-FLD) with photochemical derivatization was used. Mycotoxins were extracted from maize using a QuEChERS-based extraction procedure. The optimized analytical conditions were evaluated in terms of recoveries, reproducibility, LOD, LOQ and linearity for aflatoxin B1 and aflatoxin G1 in maize. Extraction, chromatographic and detection conditions were optimized in order to increase sample sensitivity. The linearity was analyzed in the range of 0.4-20 μg/kg and the correlation coefficients (R2 ) were higher than 0.99 for af latoxins B1 and G1. Blank samples were spiked at 1.0, 2.0 and 4.0 μg/kg, and the average recovery for aflatoxin G1 was 96.96±1.72% and for aflatoxin B1 it was 86.80±1.24%. RSDs were lower than 25% for both mycotoxins. LOD for both aflatoxins was 0.5 μg/kg and LOQ was 1.0 μg/kg, respectively. [ABSTRACT FROM AUTHOR]

Mycotoxins are highly toxic compounds produced by molds that commonly occur in cereal grains. These toxins can have adverse effects on human and animal health. Mycotoxin decontamination procedures can be divided into three different groups: chemical, biological and physical. Procedures from the first two groups are often regarded as unacceptable for lowering the mycotoxin level. A laboratory brush was developed for the purpose of physical cleaning of corn kernel. Three samples of commercially available corn kernels were subjected to the brushing procedure in order for the experimental study to be conducted. The mass of 100g of corn kernels was placed on motionless screen of the brush. A rotating part of the experimental device, the polypropylene bristle brush was set to higher speed (higher than 800 rpm). During the corn brushing, dust and broken kernels were brushed out through the motionless screen. Corn samples were taken before and after the brushing procedure and they were analyzed for aflatoxin concentration by HPLC-UV RED. By comparing the control and brushed samples, it can be noticed that removal of fines caused the reduction in the level of mycotoxins in all three brushed samples. [ABSTRACT FROM AUTHOR]