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To overcome the shortcoming of the Fuzzy C-means algorithm (FCM)—that it is easy to fall into local optima due to the dependence of sub-spatial clustering on initialization—a Multi-Strategy Tuna Swarm Optimization-Fuzzy C-means (MSTSO-FCM) algorithm is proposed. Firstly, a chaotic local search strategy and an offset distribution estimation strategy algorithm are proposed to improve the performance, enhance the population diversity of the Tuna Swarm Optimization (TSO) algorithm, and avoid falling into local optima. Secondly, the search and development characteristics of the MSTSO algorithm are introduced into the fuzzy matrix of Fuzzy C-means (FCM), which overcomes the defects of poor global searchability and sensitive initialization. Not only has the searchability of the Multi-Strategy Tuna Swarm Optimization algorithm been employed, but the fuzzy mathematical ideas of FCM have been retained, to improve the clustering accuracy, stability, and accuracy of the FCM algorithm. Finally, two sets of artificial datasets and multiple sets of the University of California Irvine (UCI) datasets are used to do the testing, and four indicators are introduced for evaluation. The results show that the MSTSO-FCM algorithm has better convergence speed than the Tuna Swarm Optimization Fuzzy C-means (TSO-FCM) algorithm, and its accuracies in the heart, liver, and iris datasets are 89.46%, 63.58%, 98.67%, respectively, which is an outstanding improvement. [ABSTRACT FROM AUTHOR]