1. Bioprocess intensification of antibiotic production by Streptomyces coelicolor A3(2) in micro-porous culture
- Author
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T.M. Ndlovu, J. Glassey, J. Eskildsen, A.C. Ward, and G. Akay
- Subjects
Materials science ,Cell Culture Techniques ,Anthraquinones ,Streptomyces coelicolor ,Bioengineering ,Bacterial Adhesion ,Actinorhodin ,Biomaterials ,Prodigiosin ,chemistry.chemical_compound ,Cell Movement ,Bioprocess ,Porosity ,Cell Proliferation ,biology ,Penetration (firestop) ,biology.organism_classification ,Antibiotic production ,Anti-Bacterial Agents ,chemistry ,Chemical engineering ,Mechanics of Materials ,Microscopy, Electron, Scanning ,Surface modification - Abstract
A novel functionalized micro-porous matrix was developed with well-controlled physicochemical proprieties such as pore size and surface chemistry. The matrix was used as a solid support in the growth of “ Streptomyces coelicolor ” A3(2) to enhance the production of antibiotics. The results shown support a higher production of prodigiosin and actinorhodin with overall production increase of 2–5 and 6–17, respectively, compared to conventional submerged liquid culture, offering a potential improvement in volumetric productivity. Scanning Electron Microscopy was used to evaluate pore size as well as bacterial adhesion, penetration, proliferation and migration within the micro-porous matrix.
- Published
- 2015
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