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Mechanical properties are crucial parameters for scaffold design for bone tissue engineering; therefore, it is important to understand the definitions of the mechanical properties of bones and relevant analysis methods, such that tissue engineers can use this information to properly design the mechanical properties of scaffolds for bone tissue engineering. The main purpose of this article is to provide a review and practical guide to understand and analyze the mechanical properties of compact bone that can be defined and extracted from the stress–strain curve measured using uniaxial tensile test until failure. The typical stress–strain curve of compact bone measured using uniaxial tensile test until failure is a bilinear, monotonically increasing curve. The associated mechanical properties can be obtained by analyzing this bilinear stress–strain curve. In this article, a computer programming code for analyzing the bilinear stress–strain curve of compact bone for quantifying the associated mechanical properties is provided, such that the readers can use this computer code to perform the analysis directly. In addition to being applied to compact bone, the information provided by this article can also be applied to quantify the mechanical properties of any material having a bilinear stress–strain curve, such as a whole bone, some metals and biomaterials. The information provided by this article can be applied by tissue engineers, such that they can have a reference to properly design the mechanical properties of scaffolds for bone tissue engineering. The information can also be applied by researchers in biomechanics and orthopedics to compare the mechanical properties of bones in different physiological or pathological conditions.

Most biomaterials and tissues are viscoelastic; thus, evaluating viscoelastic properties is important for numerous biomedical applications. Compressional viscoelastography is an ultrasound imaging technique used for measuring the viscoelastic properties of biomaterials and tissues. It analyzes the creep behavior of a material under an external mechanical compression. The aim of this study is to use finite element analysis to investigate how loading conditions (the distribution of the applied compressional pressure on the surface of the sample) and boundary conditions (the fixation method used to stabilize the sample) can affect the measurement accuracy of compressional viscoelastography. The results show that loading and boundary conditions in computational simulations of compressional viscoelastography can severely affect the measurement accuracy of the viscoelastic properties of materials. The measurement can only be accurate if the compressional pressure is exerted on the entire top surface of the sample, as well as if the bottom of the sample is fixed only along the vertical direction. These findings imply that, in an experimental validation study, the phantom design should take into account that the surface area of the pressure plate must be equal to or larger than that of the top surface of the sample, and the sample should be placed directly on the testing platform without any fixation (such as a sample container). The findings indicate that when applying compressional viscoelastography to real tissues in vivo, consideration should be given to the representative loading and boundary conditions. The findings of the present simulation study will provide a reference for experimental phantom designs regarding loading and boundary conditions, as well as guidance towards validating the experimental results of compressional viscoelastography.

Several ultrasound-based methods have been developed to evaluate the viscoelastic properties of materials. The purpose of this study is to introduce a novel viscoelastography method based on ultrasound acoustic radiation force for measuring the parameters relevant to the viscoelastic properties of materials, named ramp-creep ultrasound viscoelastography (RC viscoelastography). RC viscoelastography uses two different ultrasound excitation modes to cause ramp and creep strain responses in the material. By combining and analyzing the information obtained from these two modes of excitation, the viscoelastic parameters of the material can be quantitatively evaluated. Finite element computer simulation demonstrated that RC viscoelastography can accurately evaluate the viscoelastic parameters of the material, including the relaxation and creep time constants as well as the ratio of viscous fluids to solids in the material, except for the region near the top surface of the material. The novelty of RC viscoelastography is that there is no need to know the magnitude of acoustic radiation force and induced stress in the material in order to evaluate the viscoelastic parameters. In the future, experiments are necessary to test the performance of RC viscoelastography in real biomaterials and biological tissues.