Search

Your search keyword '"Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM)"' showing total 15 results
Start Over Author "Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM)" Journal marine environmental research
15 results on '"Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM)"'

1. Physiological adaptation of the diatom Pseudo-nitzschia delicatissima under copper starvation

Author

Marc Long, Aurélie Lelong, Eva Bucciarelli, Fabienne Le Grand, Hélène Hégaret, Philippe Soudant, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
photosynthesis, copper starvation, energy storage, photosystem II, General Medicine, Aquatic Science, Oceanography, Pollution, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, membranes, bacillariophyta, physiology, lipid metabolism, [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography
Abstract

In the open ocean and particularly in iron (Fe)-limited environment, copper (Cu) deficiency might limit the growth of phytoplankton species. Cu is an essential trace metal used in electron-transfer reactions, such as respiration and photosynthesis, when bound to specific enzymes. Some phytoplankton species, such as the diatom Pseudo-nitzschia spp. can cope with Cu starvation through adaptative strategies. In this study, we investigated the physiological strategies of the marine diatom P. delicatissima against Cu starvation. Compared to the control, Cu starvation inhibited growth by 35%, but did not induce any excess mortality. Despite the bottleneck measured in the electron flow of the photosynthetic chain, cells of P. delicatissima conserved their photosynthesis ability. This photosynthesis maintenance was accompanied by structural changes of membranes, where pigments and lipid composition were strongly modified. Diatoms also strongly modified their metabolism, by redirecting their C allocation to energy storage under the form of triglycerides. By maintaining essential metabolic functions and storing energy under the form of lipids, these physiological adaptations might be a strategy enabling this diatom to later bloom under the return of favorable nutritional condition.

Published
2023
Full Text
View/download PDF

2. Harsh intertidal environment enhances metabolism and immunity in oyster (Crassostrea gigas) spat

Author

Charlotte Corporeau, Sébastien Petton, Romain Vilaça, Lizenn Delisle, Claudie Quéré, Valérian Le Roy, Christine Dubreuil, Sandra Lacas-Gervais, Yann Guitton, Sébastien Artigaud, Benoît Bernay, Vianney Pichereau, Arnaud Huvet, Bruno Petton, Fabrice Pernet, Elodie Fleury, Stéphanie Madec, Christophe Brigaudeau, Catherine Brenner, Nathalie M. Mazure, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre Commun de Microscopie Appliquée (CCMA), Université de Nice Sophia-Antipolis (UNSA), Laboratoire d'étude des Résidus et Contaminants dans les Aliments (LABERCA), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Interactions Cellules Organismes Environnement (ICORE), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN), Lymphocytes B, Autoimmunité et Immunothérapies (LBAI), Université de Brest (UBO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-LabEX IGO Immunothérapie Grand Ouest, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO), Aspects métaboliques et systémiques de l'oncogénèse pour de nouvelles approches thérapeutiques (METSY), Institut Gustave Roussy (IGR)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Centre méditerranéen de médecine moléculaire (C3M), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA), and mazure, nathalie

Subjects
Proteomics, OsHV-1, [SDV]Life Sciences [q-bio], Marine invertebrate, General Medicine, Environment, Aquatic Science, Oceanography, Pollution, Immunity, Innate, [SDV] Life Sciences [q-bio], Metabolism, Host-Pathogen Interactions, Animals, Crassostrea, Herpesviridae
Abstract

International audience; The Pacific oyster Crassostrea gigas is established in the marine intertidal zone, experiencing rapid and highly dynamic environmental changes throughout the tidal cycle. Depending on the bathymetry, oysters face oxygen deprivation, lack of nutrients, and high changes in temperature during alternation of the cycles of emersion/ immersion. Here we showed that intertidal oysters at a bathymetry level of 3 and 5 m delayed by ten days the onset of mortality associated with Pacific Oyster Mortality Syndrome (POMS) as compared to subtidal oysters. Intertidal oysters presented a lower growth but similar energetic reserves to subtidal oysters but induced proteomic changes indicative of a boost in metabolism, inflammation, and innate immunity that may have improved their resistance during infection with the Ostreid herpes virus. Our work highlights that intertidal harsh environmental conditions modify host-pathogen interaction and improve oyster health. This study opens new perspectives on oyster farming for mitigation strategies based on tidal height.

Published
2022
Full Text
View/download PDF

3. Spatial and ontogenetic variations in sardine feeding conditions in the Bay of Biscay through fatty acid composition

Author

Philippe Soudant, Christophe Lebigre, Mathilde Bertrand, Pablo Brosset, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO), Unité de recherche Sciences et Technologies Halieutiques (STH), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Écologie et santé des écosystèmes (ESE), AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ANR-17-EURE-0015,ISBlue,Interdisciplinary Graduate School for the Blue planet(2017), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-AGROCAMPUS OUEST, and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
0106 biological sciences, Ontogeny, Zoology, Small pelagic fish, Aquatic Science, Biology, Oceanography, 010603 evolutionary biology, 01 natural sciences, Size-at-age, Life history theory, Predation, Phytoplankton, Animals, Humans, 14. Life underwater, Fatty acids, NE Atlantic, Sardina pilchardus, chemistry.chemical_classification, 010604 marine biology & hydrobiology, Sardine, Fatty Acids, Fishes, Fatty acid, General Medicine, Pollution, Lipids, chemistry, Bays, Seafood, [SDE]Environmental Sciences, Composition (visual arts), Bay
Abstract

International audience; Food characteristics are amongst the most influential factors determining the fish life history traits as quantitative and qualitative changes in individuals' diet can lead to a decline in the energy allocated to their growth, and hence influence natural populations' characteristics. The size-at-age and weight of European sardines (Sardina pilchardus) in the Bay of Biscay (BoB) have decreased substantially over the last decade, especially for the youngest age classes, and the factors underlying such changes have not yet been identified. We therefore analysed the fatty acid (FA) composition in the neutral (NL) and polar (PL) lipids in samples collected across the BoB to determine whether the diet of sardines changes with their ages. We found that the total FA contents in both lipid fractions varied mainly with the sampling locations and age. Indeed, sardines aged 1 and 2 years living in South BoB had particularly high contents in FA specific to non-diatom phytoplankton, while older sardines living in the Northern part had higher total FA content and more FA specific to copepods. These differences probably resulted from differences in prey availability and to a lesser extend a change in feeding behaviour with age. The strong dependence of younger sardines’ diet to phytoplankton in spring suggests that changes in primary production may explain their decline in size-at-age. Finally, NL clearly reflect finest feeding variations in comparison to PL imprinted by diet variations at longer time scale. Future studies should consider separately NL and PL fractions.

Published
2021
Full Text
View/download PDF

4. Changes in lipid and fatty acid contents of gonad during the reproductive cycle of the Mediterranean swordfish Xiphias gladius

Author

Fany Sardenne, Remi Millot, Elodie Bessis, Eleonora Puccinelli, Antoine Bideau, Fabienne Le Grand, Éric Dominique Henri Durieux, Philippe Soudant, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Stella Mare, Université Pascal Paoli (UPP)-Centre National de la Recherche Scientifique (CNRS), and ANR-17-EURE-0015,ISBlue,Interdisciplinary Graduate School for the Blue planet(2017)

Subjects
Male, human food source, [SDV]Life Sciences [q-bio], Reproduction, Fatty Acids, Fishes, large pelagic fish, essentials fatty acids, General Medicine, testis, Aquatic Science, Oceanography, Pollution, Perciformes, Seafood, Animals, Humans, Female, ovaries, omega-3, maturity, Gonads
Abstract

International audience; Swordfish Xiphias gladius is a large pelagic fish distributed worldwide and exploited for human consumption, however there is limited knowledge about its reproductive biology, especially regarding lipid dynamic in gonads. In teleost fish, reproductive success and offspring survival are associated to lipid availability for gamete synthesis. This study investigated the lipid composition, including lipid classes and fatty acids (FA) of cell membrane and reserve lipids (i.e., polar and neutral lipids, respectively; PL and NL), along female and male gonad development of a swordfish population from waters surrounding Corsica Island in the Mediterranean Sea. Overall, swordfish gonads contained 20% of total FA), while 20:5n-3 and 20:4n-6 were minor (3-6% of total FA) and varied little with maturation. 22:6n-3 and 18:1n-9 were selectively allocated to the ovarian maturation (increased in concentration and in proportion with maturation) until spawning. Finally, swordfish gonads might represent a good food source for humans given that 150 g of swordfish ovaries can cover the daily requirements in omega-3 for humans, but research on pollutants should also be conducted to evaluate their implications on the reproduction output of this species, and on the safety of swordfish gonads for human consumption.

Published
2022
Full Text
View/download PDF

5. The relationship between membrane fatty acid content and mitochondrial efficiency differs within- and between- omega-3 dietary treatments

Author

Philippe Soudant, Emmanuel Dubillot, Margaux Mathieu-Resuge, Marie Vagner, Nicolas Graziano, Fabienne Le Grand, Karine Salin, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), WasserCluster Lunz, Interuniversity Center for Aquatic Ecosystem Research, LIttoral ENvironnement et Sociétés (LIENSs), La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS), This work is a contribution to the project ECONAT Axe 1 - Ressources Marines Littorales: qualité et éco-valorisation, funded by the Contrat de Plan Etat-Région and the CNRS and the European Regional Development Fund., Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne), LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs), and Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, acl, [SDV]Life Sciences [q-bio], ATP/O ratio, Aquatic Science, Mitochondrion, food quality, Oceanography, 010603 evolutionary biology, 01 natural sciences, Food chain, Nutrient, Fatty Acids, Omega-3, Docosahexaenoic acid (DHA), Animals, Humans, 14. Life underwater, Food science, Global change, eicosapentaenoic acid (EPA), global change, Eicosapentaenoic acid (EPA), chemistry.chemical_classification, biology, Chemistry, Consumer, Chelon auratus, 010604 marine biology & hydrobiology, Fatty Acids, Fatty acid, General Medicine, biology.organism_classification, Pollution, Eicosapentaenoic acid, Diet, Mitochondria, Eicosapentaenoic Acid, 13. Climate action, Docosahexaenoic acid, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Golden grey mullet, Food quality
Abstract

International audience; An important, but underappreciated, consequence of climate change is the reduction in crucial nutrient production at the base of the marine food chain: the long-chain omega-3 highly unsaturated fatty acids (n-3 HUFA). This can have dramatic consequences on consumers, such as fish as they have limited capacity to synthesise n-3 HUFA de novo. The n-3 HUFA, such as docosahexaenoic acid (DHA, 22:6n-3) and eicosapentaenoic acid (EPA, 20:5n-3), are critical for the structure and function of all biological membranes. There is increasing evidence that fish will be badly affected by reductions in n-3 HUFA dietary availability, however the underlying mechanisms remain obscure. Hypotheses for how mitochondrial function should change with dietary n-3 HUFA availability have generally ignored ATP production, despite its importance to a cell's total energetics capacity, and in turn, whole-animal performance. Here we (i) quantified individual variation in mitochondrial efficiency (ATP/O ratio) of muscle and (ii) examined its relationship with content in EPA and DHA in muscle membrane of a primary consumer fish, the golden grey mullet Chelon auratus, receiving either a high or low n-3 HUFA diet. Mitochondria of fish fed on the low n-3 HUFA diet had higher ATP/O ratio than those of fish maintained on the high n-3 HUFA diet. Yet, mitochondrial efficiency varied up about 2-fold among individuals on the same dietary treatment, resulting in some fish consuming half the oxygen and energy substrate to produce the similar amount of ATP than conspecific on similar diet. This variation in mitochondrial efficiency among individuals from the same diet treatment was related to individual differences in fatty acid composition of the membranes: a high ATP/O ratio was associated with a high content in EPA and DHA in biological membranes. Our results highlight the existence of interindividual differences in mitochondrial efficiency and its potential importance in explaining intraspecific variation in response to food chain changes.

Published
2020
Full Text
View/download PDF

6. Effect of long-term intergenerational exposure to ocean acidification on ompa and ompb transcripts expression in European seabass (Dicentrarchus labrax)

Author

Arianna Servili, Carolin J. Neven, Felix Christopher Mark, Sophie Collet, Jose L. Zambonino-Infante, Lauriane Madec, Thomas Vitré, David Mazurais, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), ANR-10-LABX-0019,LabexMER,LabexMER Marine Excellence Research: a changing ocean(2010), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, Olfactory system, Oceans and Seas, Aquatic Science, Oceanography, 010603 evolutionary biology, 01 natural sciences, Genome, Acidification, 03 medical and health sciences, Intergenerational, medicine, Animals, Seawater, Expression pattern, Dicentrarchus labrax, 14. Life underwater, Gene, 030304 developmental biology, [SDU.OCEAN]Sciences of the Universe [physics]/Ocean, Atmosphere, chemistry.chemical_classification, 0303 health sciences, Messenger RNA, biology, General Medicine, Hydrogen-Ion Concentration, bacterial infections and mycoses, biology.organism_classification, OMP, Pollution, Cell biology, Amino acid, medicine.anatomical_structure, Seafood, chemistry, [SDE]Environmental Sciences, biology.protein, bacteria, Bass, Dicentrarchus, Olfactory epithelium, Olfactory marker protein
Abstract

International audience; Since sensory system allows organisms to perceive and interact with their external environment, any disruption in their functioning may have detrimental consequences on their survival. Ocean acidification has been shown to potentially impair olfactory system in fish and it is therefore essential to develop biological tools contributing to better characterize such effects. The olfactory marker protein (omp) gene is involved in the maturation and the activity of olfactory sensory neurons in vertebrates. In teleosts, two omp genes (ompa and ompb) originating from whole genome duplication have been identified. In this study, bioinformatic analysis allowed characterization of the ompa and ompb genes from the European seabass (Dicentrarchus labrax) genome. The European seabass ompa and ompb genes differ in deduced amino acid sequences and in their expression pattern throughout the tissues. While both ompa and ompb mRNA are strongly expressed in the olfactory epithelium, ompb expression was further observable in different brain areas while ompa expression was also detected in the eyes and in other peripheral tissues. Expression levels of ompa and ompb mRNA were investigated in adult seabass (4 years-old, F0) and in their offspring (F1) exposed to pH of 8 (control) or 7.6 (ocean acidification, OA). Under OA ompb mRNA was down-regulated while ompa mRNA was up-regulated in the olfactory epithelium of F0 adults, suggesting a long-term intragenerational OA-induced regulation of the olfactory sensory system. A shift in the expression profiles of both ompa and ompb mRNA was observed at early larval stages in F1 under OA, suggesting a disruption in the developmental process. Contrary to the F0, the expression of ompa and ompb mRNA was not anymore significantly regulated under OA in the olfactory epithelium of juvenile F1 fish. This work provides evidence for long-term impact of OA on sensorial system of European seabass as well as potential intergenerational acclimation of omp genes expression to OA in European seabass.

Published
2021
Full Text
View/download PDF

7. Is starvation a cause of overmortality of the Mediterranean sardine?

Author

Eric Gasset, Quentin Queiros, Amandine Marguerite, Jean-Marc Fromentin, David J. McKenzie, Claire Saraux, Gilbert Dutto, Pablo Brosset, MARine Biodiversity Exploitation and Conservation (UMR MARBEC), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut de Recherche pour le Développement (IRD), Département Ecologie, Physiologie et Ethologie (DEPE-IPHC), Institut Pluridisciplinaire Hubert Curien (IPHC), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Service d' Experimentations Aquacoles [Palavas les Flots] (LSEA MARBEC), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut de Recherche pour le Développement (IRD)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut de Recherche pour le Développement (IRD), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Interdisciplinary Graduate School for the Blue planet, ANR-17-EURE-0015,ISBlue,Interdisciplinary Graduate School for the Blue planet(2017), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, Mediterranean climate, Population Dynamics, Population, Zoology, Phenotypic plasticity, Small pelagic fish, Aquatic Science, Biology, Oceanography, 010603 evolutionary biology, 01 natural sciences, Mediterranean Sea, Animal mortality, medicine, Animals, Humans, 14. Life underwater, education, Population dynamics of fisheries, Starvation, Larva, education.field_of_study, Body condition, Sardine, 010604 marine biology & hydrobiology, Natural mortality, Fishes, Fasting, General Medicine, Pollution, Seafood, [SDU]Sciences of the Universe [physics], [SDE]Environmental Sciences, Seasons, medicine.symptom, Experiments
Abstract

International audience; Animal mortality is difficult to observe in marine systems, preventing a mechanistic understanding of major drivers of fish population dynamics. In particular, starvation is known to be a major cause of mortality at larval stages, but adult mortality is often unknown. In this study, we used a laboratory food-deprivation experiment, on wild caught sardine Sardina pilchardus from the Gulf of Lions. This population is interesting because mean individual phenotype shifted around 2008, becoming dominated by small, young individuals in poor body condition, a phenomenon that may result from declines in energy availability. Continuous monitoring of body mass loss and metabolic rate in 78 captive food-deprived individuals revealed that sardines could survive for up to 57 days on body reserves. Sardines submitted to long-term caloric restriction prior to food-deprivation displayed adaptive phenotypic plasticity, reducing metabolic energy expenditure and enduring starvation for longer than sardines that had not been calorie-restricted. Overall, entry into critical fasting phase 3 occurred at a body condition of 0.72. Such a degree of leanness has rarely been observed over 34 years of wild population monitoring. Still, the proportion of sardines below this threshold has doubled since 2008 and is maximal in January and February (the peak of the reproductive season), now reaching almost 10 % of the population at that time. These results indicate that the demographic changes observed in the wild may result in part from starvation-related adult mortality at the end of the winter reproductive period, despite adaptive plastic responses.

Published
2021
Full Text
View/download PDF

8. Multiple effects of a Gracilaria vermiculophylla invasion on estuarine mudflat functioning and diversity

Author

Valérie Stiger-Pouvreau, Pascal Riera, Doriane Stagnol, Nathalie Poupart, T. Androuin, Dominique Davoult, Fanny Noisette, Gwladys Surget, Ecogéochimie et Fonctionnement des Ecosystèmes Benthiques (EFEB), Adaptation et diversité en milieu marin (AD2M), Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), ANR-12-SEAS-0002,INVASIVES,Invasive seaweeds in rising temperatures: impacts and risk assessments(2012), Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO), Adaptation et diversité en milieu marin (ADMM), Institut national des sciences de l'Univers (INSU - CNRS)-Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, Invasive, Meiobenthos, Population, Aquatic Science, Biology, Oceanography, 010603 evolutionary biology, 01 natural sciences, [SDV.EE.ECO]Life Sciences [q-bio]/Ecology, environment/Ecosystems, Abundance (ecology), Macrofauna diversity, Gracilaria, Meiofauna diversity, Ecosystem, 14. Life underwater, education, Red alga, Biomass (ecology), education.field_of_study, Primary producers, Ecology, ACL, 010604 marine biology & hydrobiology, Primary production, Food web, General Medicine, 15. Life on land, Engineer species, Pollution, Europe, Fishery, Metabolism, Non-indigenous species, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Estuaries, Introduced Species, Environmental Monitoring, [SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis
Abstract

International audience; The invasive Japanese seaweed Gracilaria vermiculophylla has become established over the past several years in numerous European estuaries, from Portugal to Norway. In the Faou estuary (48.295°N-4.179°W, Brittany, France), it forms a dense population at the mud's surface. The effects of G. vermiculophylla on metabolism, diversity, and the food web were studied. Community gross primary production (GPP) and respiration (CR) during emersion, chlorophyll-a content, macrofaunal and meiofaunal diversity and abundance, and stable isotopes (δ13C and δ15N) of representative macrofaunal species and main food sources were measured at low tide in winter, spring, summer 2014, and winter 2015. Results show significant seasonal variation in GPP and CR. Moreover, GPP was significantly higher in areas where G. vermiculophylla was present than in the control area (bare mud). However, this high GPP appeared to be linked to the increase in biomass in primary producers, with their efficiency (primary productivity, i.e. assimilation number) remaining relatively stable compared with the control area. Significant variation in abundance of meiofauna and macrofauna was also detected and new epifaunal species were collected, mainly in Gracilaria-colonized areas. Isotopic food-web Bayesian mixing models strongly suggested that G. vermiculophylla plays a major role in the diet of some dominant species. Mechanisms interacting with the functioning and diversity of the mudflat are discussed. Finally, the invasive seaweed G. vermiculophylla affected the mudflat ecosystem in three ways: as a new primary producer (increase in metabolism), as a habitat-forming species (changes in diversity and abundance of macrofauna and meiofauna), and as a new abundant food source, likely through the detrital pathway.

Published
2017
Full Text
View/download PDF

9. A current synthesis on the effects of electric and magnetic fields emitted by submarine power cables on invertebrates

Author

Aurélie Jolivet, Frédéric Olivier, Luana Albert, François Deschamps, Sylvain Chauvaud, Laurent Chauvaud, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), TBM environnement, Réseau de Transport d'Electricité [Paris] (RTE), Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Station de Biologie Marine de Concarneau, Direction générale déléguée à la Recherche, à l’Expertise, à la Valorisation et à l’Enseignement-Formation (DGD.REVE), Muséum national d'Histoire naturelle (MNHN)-Muséum national d'Histoire naturelle (MNHN), This research was funded by the OASICE project, Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), and Muséum national d'Histoire naturelle (MNHN)

Subjects
0106 biological sciences, Aquatic Organisms, Electric fields, Field (physics), Earth science, Aquatic Science, Oceanography, 010603 evolutionary biology, 01 natural sciences, Submarine power cables, Electric field, Animals, Renewable Energy, 14. Life underwater, Sediment compartment, Magneto, Ships, [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography, Electroreception, Marine invertebrates, business.industry, ACL, 010604 marine biology & hydrobiology, Submarine, General Medicine, Invertebrates, Pollution, Renewable energy, Magnetic field, Benthic compartment, 13. Climate action, Magnetic fields, [SDE]Environmental Sciences, Environmental science, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, business
Abstract

International audience; The goal of clean renewable energy production has promoted the large-scale deployment of marine renewable energy devices, and their associated submarine cable network. Power cables produce both electric and magnetic fields that raise environmental concerns as many marine organisms have magneto and electroreception abilities used for vital purposes. Magnetic and electric fields’ intensities decrease with distance away from the cable. Accordingly, the benthic and the sedimentary compartments are exposed to the highest field values. Although marine invertebrate species are the major fauna of these potentially exposed areas, they have so far received little attention. We provide extensive background knowledge on natural and anthropogenic marine sources of magnetic and electric fields. We then compile evidence for magneto- and electro-sensitivity in marine invertebrates and further highlight what is currently known about their interactions with artificial sources of magnetic and electric fields. Finally we discuss the main gaps and future challenges that require further investigation.

Published
2020
Full Text
View/download PDF

10. Transgenerational regulation of cbln11 gene expression in the olfactory rosette of the European sea bass (Dicentrarchus labrax) exposed to ocean acidification

Author

Thomas Vitré, Maelenn Le Roy, Lauriane Madec, Cyril Noël, Alexandre Cormier, David Mazurais, José-Luis Zambonino-Infante, Arianna Servili, Sophie Collet, Romane Leseur, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), LabexMer (OASYS project) [ANR-10LABX-0019], Ministry of Ecological and Solidarity Transition, Foundation for Biodiversity Research (Ocean Acidification Program, PACIO project), Deutsche ForschungemeinschaftGerman Research Foundation (DFG) [PE 1157/8-1, MA4271/3-1], ANR-10-LABX-0019,LabexMER,LabexMER Marine Excellence Research: a changing ocean(2010), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), and Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne)

Subjects
IMPACTS, 0106 biological sciences, MARINE FISH, Danio, Gene Expression, RAINBOW-TROUT, PROTEIN, Zoology, Aquatic Science, Oceanography, 010603 evolutionary biology, 01 natural sciences, CARBON-DIOXIDE, Gene expression, medicine, Animals, IMMUNE-RESPONSE, Seawater, Amino Acid Sequence, 14. Life underwater, Sea bass, PACIFIC OYSTER, Zebrafish, Regulation of gene expression, IDENTIFICATION, biology, ACL, 010604 marine biology & hydrobiology, General Medicine, Hydrogen-Ion Concentration, biology.organism_classification, Pollution, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, medicine.anatomical_structure, Gene Expression Regulation, Intercellular Signaling Peptides and Proteins, Bass, Dicentrarchus, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, ELEVATED CO2, Olfactory epithelium, C1Q, Orthologous Gene
Abstract

International audience; Elevated amounts of atmospheric CO2 are causing ocean acidification (OA) that may affect marine organisms including fish species. While several studies carried out in fish revealed that OA induces short term dysfunction in sensory systems including regulation of neurons activity in olfactory epithelium, information on the effects of OA on other physiological processes and actors is scarcer. In the present study we focused our attention on a European sea bass (Dicentrarchus labrax) sghC1q gene, a member of the C1q-domain-containing (C1qDC) protein family. In vertebrates, C1qDC family includes actors involved in different physiological processes including immune response and synaptic organization. Our microsynteny analysis revealed that this sghC1q gene is the orthologous gene in European sea bass to zebrafish (Danio rerio) cbln11 gene. We cloned the full length cbln11 mRNA and identified the different domains (the signal peptide, the coiled coil region and the globular C1q domain) of the deduced protein sequence. Investigation of mRNA expression by qPCR and in situ hybridization revealed that cbln11 gene is especially expressed in the non-sensory epithelium of the olfactory rosette at larval and adult stages. The expression of cbln11 mRNA was analysed by qPCR in the first generation (FO) of European sea bass broodstock exposed since larval stages to water pH of 8.0 (control) or 7.6 (predicted for year 2100) and in their offspring (F1) maintained in the environmental conditions of their parents. Our results showed that cbln11 mRNA expression level was lower in larvae exposed to OA then up-regulated at adult stage in the olfactory rosette of F0 and that this up-regulation is maintained under OA at larval and juvenile stages in F1. Overall, this work provides evidence of a transgenerational inheritance of OA-induced up-regulation of cbln11 gene expression in European sea bass. Further studies will investigate the potential immune function of cbln11 gene and the consequences of these regulations, as well as the possible implications in terms of fitness and adaptation to OA in European sea bass.

Published
2020
Full Text
View/download PDF

11. Evaluation of the impact of polyethylene microbeads ingestion in European sea bass (Dicentrarchus labrax) larvae

Author

Lauriane Madec, José-Luis Zambonino-Infante, Patrick Quazuguel, Olivier Mouchel, Arnaud Huvet, David Mazurais, Christine Huelvan, Bruno Ernande, Armelle Severe, Johan Robbens, Philippe Soudant, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), and Laboratoire Ressources halieutiques Manche Mer du nord, IFREMER Centre Manche Mer du Nord, (HMMN)

Subjects
Fish Proteins, Microplastics, Survival, Interleukin-1beta, Longevity, Aquatic Science, Oceanography, Fish larvae, Larvae, Animal science, Cytochrome P-450 CYP1A1, Animals, Ingestion, 14. Life underwater, Sea bass, Trophic level, Larva, Gastrointestinal tract, biology, ACL, Microplastic, fungi, General Medicine, biology.organism_classification, Pollution, Microspheres, Fishery, Fish, Polyethylene, Animal Nutritional Physiological Phenomena, Bass, Digestion, Dicentrarchus, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Water Pollutants, Chemical
Abstract

Microplastics are present in marine habitats worldwide and may be ingested by low trophic organisms such as fish larvae, with uncertain physiological consequences. The present study aims at assessing the impact of polyethylene (PE 10-45 mu M) microbeads ingestion in European sea bass (Dicentrarchus labrax) larvae. Fish were fed an inert diet including 0, 10(4) and 10(5) fluorescent microbeads per gram from 7 until 43 days post-hatching (dph). Microbeads were detected in the gastrointestinal tract in all fish fed diet incorporating PE. Our data revealed an efficient elimination of PE beads from the gut since no fluorescent was observed in the larvae after 48 h depuration. While the mortality rate increased significantly with the amount of microbeads scored per larvae at 14 and 20 dph, only ingestion of the highest concentration slightly impacted mortality rates. Larval growth and inflammatory response through Interleukine-1-beta (IL-1 beta) gene expression were not found to be affected while cytochrome-P450-1A1 (cyp1a1) expression level was significantly positively correlated with the number of microbeads scored per larva at 20 dph. Overall, these results suggest that ingestion of PE microbeads had limited impact on sea bass larvae possibly due to their high potential of egestion.

Published
2015
Full Text
View/download PDF

12. Genetic polymorphism and its potential relation to environmental stress in five populations of the European flounder Platichthys flesus, along the French Atlantic coast

Author

Jean Laroche, Justine Marchand, Louis Quiniou, Estérine Evrard, Bruno Guinand, Jérôme Cachot, Le Mans Université (UM), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Université Sciences et Technologies - Bordeaux 1 (UB), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE), and Université Sciences et Technologies - Bordeaux 1

Subjects
selection, Flounder, 010501 environmental sciences, Aquatic Science, Pleuronectidae, Oceanography, Polymorphism, Single Nucleotide, 01 natural sciences, polymorphism, 03 medical and health sciences, contamination, Gene Frequency, Stress, Physiological, Polymorphism (computer science), Animals, 14. Life underwater, EUROPEAN FLOUNDER, gene, Atlantic Ocean, Creatine Kinase, Allele frequency, 030304 developmental biology, 0105 earth and related environmental sciences, Genetics, 0303 health sciences, biology, Glyceraldehyde-3-Phosphate Dehydrogenases, Single-strand conformation polymorphism, General Medicine, biology.organism_classification, Pollution, Platichthys, Lipocalins, Intramolecular Oxidoreductases, Betaine-Homocysteine S-Methyltransferase, Genetic marker, France, PCR-SSCP, Environmental Monitoring
Abstract

In this study, new DNA markers were explored for the flounder Platichthys flesus. cDNA and genomic sequences of the genes encoding the glyceraldehyde-3-phosphate-deshydrogenase (GAPDH), the cytosolic creatine kinase (CK), the prostaglandin D synthase (PGDS) and the betaine homocysteine methyltransferase (BHMT) were characterized. The tumour suppressor p53 gene structure was already described. A PCR–SSCP (Single Strand Conformation Polymorphism) analysis was finally conducted to study the genetic polymorphism of different populations of flounders collected along the French Atlantic coast. Four highly contaminated French estuaries (Seine, Vilaine, Loire and Gironde) were sampled and compared to a reference estuary (Ster) to explore possible selective effect of the environment on specific allelic frequencies. Our results showed that two loci p53 and PGDS, could be potential markers of chemical stress: p53A allele frequency increased in contaminated systems compared to the reference system. In the Vilaine estuary, PGDS polymorphism could be related to pesticide stress.

Published
2010
Full Text
View/download PDF

13. Endoliths in Lithophaga lithophaga shells--Variation in intensity of infestation and species occurrence

Author

Jasna Arapov, Barbara Calcinai, Melita Peharda, Julien Thébault, Sanja Puljas, Stjepko Golubic, Università Politecnica delle Marche [Ancona] (UNIVPM), University of Split, Boston University [Boston] (BU), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Lithophaga lithophaga, Adriatic Sea, Oceans and Seas, Aquatic Science, Biology, Mediterranean, Oceanography, Coastal zone, marine ecology, endoliths, sclerochronology, eutrophication, Marine ecology, temporal trends, Sclerochronology, Animals, patterns, 14. Life underwater, Lithophaga, 1758 bivalvia, northern tunisia, date mussel, Population Density, geography, geography.geographical_feature_category, Mediterranean Region, ACL, General Medicine, Coral reef, Biodiversity, Eutrophication, biology.organism_classification, Pollution, Porifera, bioerosion rates, coral-reefs, Mytilidae, Productivity (ecology), Pione vastifica, great-barrier-reef, reproductive-cycle, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Bay, Environmental Monitoring
Abstract

WOS:000356545300009; Pronounced differences with respect to the extent of infestation and the degree of Lithophaga lithophaga shell damage inflicted by euendolithic taxa at two sites in the Adriatic Sea representing different productivity conditions, are described. Shells collected from the eastern part of Kastela Bay, which is characterized by higher primary productivity, have significantly more shell damage then the shell collected from a site on the outer coast of the island of Ciovo exposed to the oligotrophic Adriatic Sea. The presence of endoliths and their perforations were detected in different layers of the shell, including solidly mineralized parts of the skeleton and within the organic lamellae incorporated into the shell. Phototrophic endoliths were not observed in the specimens. The most serious damage to L. lithophaga shells was the boring clionaid sponge Pione vastifica, which was more common in shells collected from Kastela.

Published
2015
Full Text
View/download PDF

14. Phenology, TPC and size-fractioning phenolics variability in temperate Sargassaceae (Phaeophyceae, Fucales) from Western Brittany: native versus introduced species

Author

Solène Connan, Valérie Stiger-Pouvreau, K. Le Lann, Laboratoire d'Écophysiologie et de Biotechnologie des Halophytes et des Algues Marines (LEBHAM), Université de Brest (UBO), Institut national des sciences et techniques de la mer (INTECHMER), Conservatoire National des Arts et Métiers [CNAM] (CNAM), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, Population dynamics, Chemical defence, Aquatic Science, Cystoseira, Oceanography, Phaeophyta, 010603 evolutionary biology, 01 natural sciences, Marine ecology, Cystoseira baccata, Phenols, Species Specificity, Botany, Sargassum muticum, Phenological plasticity, biology, Phenology, 010604 marine biology & hydrobiology, Sargassum, General Medicine, 15. Life on land, biology.organism_classification, Pollution, Phenolic compounds, Bifurcaria, Alien vs. native algae, [SDE]Environmental Sciences, Sargassaceae, Seasons, Fucales, Introduced Species, Biological invasion
Abstract

International audience; The phenology of the introduced Sargassum muticum and two native species Bifurcaria bifurcata and Cystoseira baccata were monitored during eighteen months at two sites in Brittany. Density and length varied seasonally only in Sargassum. Larger individuals of Sargassum were observed in summer whereas in Cystoseira, they appeared almost in autumn. Peaks in maturity were delayed: in summer for Sargassum and in winter for Cystoseira and Bifurcaria. Phenolic contents increased before their respective reproductive period as a chemical defence. Moreover, size composition varied with site and season depending on species. In Sargassum, the quantity of small compounds decreased in summer together with an increase of 2000/5000 Da compounds. In Bifurcaria 2000/5000 Da compounds increased in summer (photoprotection) while in Cystoseira it increased in winter (protection for reproduction). Sargassum presented then a phenological plasticity not observed in native species. Moreover the three species possessed different chemical strategies to succeed in partitioning their vital space.

Published
2012
Full Text
View/download PDF

15. Specific pathways for the incorporation of dissolved barium and molybdenum into the bivalve shell: an isotopic tracer approach in the juvenile Great Scallop (Pecten maximus)

Author

Aurélie Jolivet, Laurent Chauvaud, Sébastien Pomerleau, David Amouroux, Ricardo Riso, Christophe Pécheyran, Julien Thébault, Hélène Tabouret, Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Pau et des Pays de l'Adour (UPPA), Departement de Biologie, Chimie et Géographie, Université du Québec à Rimouski (UQAR), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Gills, 010504 meteorology & atmospheric sciences, chemistry.chemical_element, Mineralogy, Aquatic Science, Biogeochemical cycle, 010502 geochemistry & geophysics, Oceanography, Mass spectrometry, 01 natural sciences, Femtosecond laser ablation, chemistry.chemical_compound, Scallop shell, Isotopes, Tracers, Pecten maximus, Animals, Seawater, Bivalve shell, 0105 earth and related environmental sciences, Calcite, Molybdenum, biology, Muscles, Radiochemistry, Barium, General Medicine, biology.organism_classification, Pollution, Pectinidae, chemistry, [SDE]Environmental Sciences, Scallop, Inductively coupled plasma
Abstract

International audience; Dissolved barium and molybdenum incorporation in the calcite shell was investigated in the Great Scallop Pecten maximus. Sixty six individuals were exposed for 16 days to two successive dissolved Ba and Mo concentrations accurately differentiated by two different isotopic enrichments (97Mo, 95Mo; 135Ba, 137Ba). Soft tissue and shell isotopic composition were determined respectively by quantitative ICP-MS (Inductively Coupled Plasma Mass Spectrometer) and laser ablation - ICP-MS. Results from Ba enrichment indicate the direct incorporation of dissolved Ba into the shell in proportion to the levels in the water in which they grew with a 6-8 day delay. The low spike contributions and the low partition coefficient (DMo = 0.0049 ± 0.0013), show that neither the soft tissue nor the shell were significantly sensitive to Mo enrichment. These results eliminate direct Mo shell enrichment by the dissolved phase, and favour a trophic uptake that will be investigated using the successive isotopic enrichment approach developed in this study.

Published
2011
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results