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The article presents a study on the diet and prey preference of two populations of juvenile lemon sharks or Negaprion brevirostris from Bimini, Bahamas. The study analyzed the stomach contents of the sharks along with data on prey species and abundance to shed light on shark's preference in terms of prey type, size, and location. Contingency table analysis was used for the spatial and temporal variations in prey abundance in lemon sharks' diet. It also shows that the dietary analysis used for the study presents that recovered prey in the stomachs of sharks were determined to have lowest taxonomic level, count, weight and measure. Results further indicate that sharks mostly feed on yellowfin mojarra, they exhibit prey preference and size selection, and have hierarchy of preference.