1. Sponge-cell culture? A molecular identification method for sponge cells
- Author
-
Ronald Osinga, Johannes Tramper, Detmer Sipkema, G.H.J. Heilig, René H. Wijffels, and A.D.L. Akkermans
- Subjects
Marine sponges ,Bio Process Engineering ,Molecular Sequence Data ,Cell Culture Techniques ,Applied Microbiology and Biotechnology ,Microbiology ,18S ribosomal RNA ,Microbiologie ,Animals ,18s rdna ,marine sponges ,Gene ,Molecular identification ,VLAG ,DNA Primers ,WIMEK ,biology ,Base Sequence ,Genetic Variation ,Sequence Analysis, DNA ,biology.organism_classification ,Dysidea avara ,Molecular biology ,Porifera ,Sponge ,Biochemistry ,cultivation ,Cell culture ,RNA, Ribosomal ,Polymorphism, Restriction Fragment Length - Abstract
Dissociated sponge cells are easily confused with unicellular organisms. This has been an obstacle in the development of sponge-cell lines. We developed a molecular detection method to identify cells of the sponge Dysidea avara in dissociated cell cultures. The 18S ribosomal RNA gene from a Dysidea avara specimen was sequenced and compared to eukaryotic 18S rDNA sequences picked up from a proliferating cell culture that originated from a dissociated Dysidea avara specimen. Our method proved unambiguously that this was not a sponge-cell culture. Therefore, it provides a valuable tool for further research on sponge-cell cultures.
- Published
- 2002