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Periodontitis is an inflammatory disease of the supporting tissue of the teeth caused by specific microorganisms or groups of specific microorganisms, resulting in progressive destruction of the periodontal ligament and alveolar bone with periodontal pocket formation. Application of ozonated olive oil in dentistry is based on the actions such as antimicrobial and therapeutic agent, needed as adjunctive therapy after periodontal pocket curretage. Collagen is the main constituent of alveolar bone extracellular matrix and needed as a scaffold in the formation of mineralized matrix. The aim of this study was to determine the density of collagen in alveolar bone on periodontitis healing process after adjunctive topical application of ozonated olive oil in periodontal pocket curretage. In this study, 32 Sprague dawley rats were randomly divided into two groups: curetted-topical application of ozonated olive oil as treatment groups and curetted-topical application of 1% CMC-Na as placebo group. Periodontitis induced by placing silk-ligature around submandibular incisors for 7 days. Subsequently, the rats were sacrificed on days 3, 5, 7, and 14 after curetted and topical application, and each group was represented by four rats. The staining was done using Mallory staining method. All the results were statistically analyzed using Kruskal-Wallis and Mann-Whitney tests. The results showed that there were significant differences (p

Ozonated olive oil has been widely used as a local infection therapy to overcome bacterial resistance from antibiotics with systemic administration. However, the disadvantage of systemic antibiotics is it cannot be used in large doses for local infection therapy. Periodontitis is an infectious disease that causes alveolar bone damage. In the periodontitis healing process, osteoblasts and angiogenesis play essential roles in bone regeneration. The study aims to determine the effect of topical application ozonated olive oil to osteoblasts number and angiogenesis of alveolar bone in periodontitis healing process using in vivo study. 32 Sprague Dawley rats were divided into two groups: the treatment and placebo groups, 16 rats in each group. The induction of periodontitis was performed by ligating lower incisor with silk ligature for seven days. The treatment group received ozonated olive oil, and the placebo group received 1 % of CMC-Na twice a day. Four rats from each group were necropsied on day 3, 5, 7, and 14 and then processed into histological sample preparations by hematoxylin-eosin staining and counted the number of osteoblast and blood vessels. All collected data were analyzed using two-way ANOVA and posthoc LSD test. The average number of osteoblasts, blood vessels in the treatment group were significantly higher than the placebo group (p