1. Structure elucidation of antiproliferative bisbenzylisoquinoline alkaloids from Anisocycla grandidieri from the Madagascar dry forest.
- Author
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Liu Y, Harinantenaina L, Brodie PJ, Slebodnick C, Callmander MW, Rakotondrajaona R, Rakotobe E, Rasamison VE, TenDyke K, Shen Y, and Kingston DG
- Subjects
- Antineoplastic Agents, Phytogenic isolation & purification, Cell Line, Tumor, Cell Proliferation drug effects, Dose-Response Relationship, Drug, Drug Screening Assays, Antitumor, Humans, MCF-7 Cells, Madagascar, Magnetic Resonance Spectroscopy standards, Models, Molecular, Molecular Structure, Plant Stems chemistry, Reference Standards, Structure-Activity Relationship, Antineoplastic Agents, Phytogenic chemistry, Antineoplastic Agents, Phytogenic pharmacology, Menispermaceae chemistry, Trees chemistry
- Abstract
Antiproliferative bioassay-guided fractionation of the ethanol extract of the stems of Anisocycla grandidieri led to the isolation of the known alkaloids stebisimine (1), (+)-1,2-dehydrotelobine (2), (+)-2'-norcocsuline (3) and puetogaline B (4). Herein, we report the full NMR assignments of all compounds and the X-ray crystallography of single crystals of compounds 1 and 3. Compounds 2 and 3 showed moderate antiproliferative activity against the A2780 human ovarian cancer cell line with IC50 values of 4.1 ± 0.3 and 2.7 ± 0.3 μM, respectively, and they also displayed selective activity toward the H460 (large cell lung cancer), MCF-7 (breast ductal carcinoma), and UACC-257 (melanoma) cell lines., (Copyright © 2013 John Wiley & Sons, Ltd.)
- Published
- 2013
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