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Three Pseudomonas fluorescensstrains and the type strain Pseudomonas putidaCFBP2066 inhibited invasion of the plant‐parasitic nematodes Radopholus similisand Meloidogynespp. in banana, maize and tomato roots. Results were, however, not always significantly different from controls. One Ps. fluorescensstrain kept R. similisnumbers significantly lower in banana roots after the initial invasion stage. All strains also showed an in vitrorepellent effect towards the nematodes, with Meloidogynespp. being more affected than R. similis.As Ps. putidaCFBP2066 was negative for the enzymatic activities tested as well as HCN productivity, it was concluded that either other chemical bacterial compounds affected nematode infectivity or strains elicited induced systemic resistance in plants.