Search

Showing total 2 results
Start Over Topic basidiomycota Journal letters in applied microbiology
2 results

1. Laccase stabilization by covalent binding immobilization on activated polyvinyl alcohol carrier.

Author

Yinghui D, Qiuling W, and Shiyu F

Subjects
Chlorophenols metabolism, Enzyme Stability, Industrial Waste, Laccase, Paper, Waste Disposal, Fluid methods, Basidiomycota enzymology, Enzymes, Immobilized, Oxidoreductases chemistry, Oxidoreductases metabolism, Polyvinyl Alcohol chemistry
Abstract

Aims: Attempts were made to immobilize laccase from Panus conchatus., Methods and Results: The laccase was immobilized on carboxylated polyvinyl alcohol (PVA) activated by N-hydroxysuccinimide (N-HSI) in aqueous solution at different pHs, temperatures, and with different reaction times. An optimum condition for laccase immobilization is at pH 3.2, 40 degrees C and 12 h, respectively. Immobilization of laccase increased optimal pH for reaction with 2, 2'-azinobis (3-ethylbenzthiazoline-6-solfonate) (ABTS) and pH stability. Immobilized laccase proved to be reacted consecutively 17 times with only a 50% decrease on activity and be used in removal of 2,4,6-trichlorophenol (TCP)., Conclusions: It was possible to immobilize the laccase on carboxylated polyvinyl alcohol by activation with N-hydroxysuccinimide in HAc-NaAc buffer. The immobilized laccase is both stable and reusable. SIGNIFICANCE IMPACT OF THE STUDY: The results indicate that this immobilized laccase can be used in the removal of poisonous effluent from pulp bleaching mills.

Published
2002
Full Text
View/download PDF

2. Laccase stabilization by covalent binding immobilization on activated polyvinyl alcohol carrier

Author

Fu Shiyu, Wang Qiuling, and Diao Yinghui

Subjects
Paper, Laccase, ABTS, Aqueous solution, Immobilized enzyme, biology, Basidiomycota, Industrial Waste, Enzymes, Immobilized, biology.organism_classification, Waste Disposal, Fluid, Applied Microbiology and Biotechnology, Polyvinyl alcohol, chemistry.chemical_compound, chemistry, Biochemistry, Covalent bond, Polyvinyl Alcohol, Enzyme Stability, Panus conchatus, Oxidoreductases, Chlorophenols, Waste disposal, Nuclear chemistry
Abstract

Aim: Attempts were made to immobilize laccase from Panus conchatus. Methods and Results: The laccase was immobilized on carboxylated polyvinyl alcohol (PVA) activated by N-hydroxysuccinimide (N-HSI) in aqueous solution at different pHs, temperatures, and with different reaction times. An optimum condition for laccase immobilization is at pH 3·2, 40 °C and 12 h, respectively. Immobilization of laccase increased optimal pH for reaction with 2, 2′-azinobis (3-ethylbenzthiazoline-6-solfonate) (ABTS) and pH stability. Immobilized laccase proved to be reacted consecutively 17 times with only a 50% decrease on activity and be used in removal of 2,4,6-trichlorophenol (TCP). Conclusions: It was possible to immobilize the laccase on carboxylated polyvinyl alcohol by activation with N-hydroxysuccinimide in HAc-NaAc buffer. The immobilized laccase is both stable and reusable. Significance and Impact of the Study: The results indicate that this immobilized laccase can be used in the removal of poisonous effluent from pulp bleaching mills.

Published
2002
Full Text
View/download PDF