1. Evaluation of gelatin particle agglutination assay for the detection of anti-PGLI antibodies. Comparison with ELISA method and applicability on a large scale study using blood collected on filter paper
- Author
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Suzanne Chanteau, J. L. Cartel, J.-F. Roux, and Jean-Paul Boutin
- Subjects
Paper ,musculoskeletal diseases ,congenital, hereditary, and neonatal diseases and abnormalities ,medicine.medical_specialty ,food.ingredient ,Enzyme-Linked Immunosorbent Assay ,Sensitivity and Specificity ,Gelatin ,Gastroenterology ,Serology ,food ,Particle agglutination ,Agglutination Tests ,Leprosy ,Internal medicine ,Direct agglutination test ,Humans ,Medicine ,Elisa method ,Antigens, Bacterial ,Blood Specimen Collection ,Filter paper ,biology ,business.industry ,General Medicine ,Antibodies, Bacterial ,Mycobacterium leprae ,Titer ,Immunology ,biology.protein ,Glycolipids ,Antibody ,business - Abstract
Given the technical difficulties of the ELISA method, a gelatin particle agglutination test (MLPA) has been developed recently for the detection of anti-PGLI antibodies. The purpose of this study was to compare these 2 tests. MLPA was found to be less specific than ELISA (91% versus 98%, chi 2 = 66.8, p less than 0.001). The sensitivity of both tests was of 95% for the diagnosis of multibacillary patients. In the case of paucibacillary patients. MLPA was found to be less sensitive than ELISA (21% versus 35%, chi 2 = 6.98, p greater than 0.01). The agreement between the 2 tests for a positive or a negative result was satisfying (85% to 100%), except for the weakly seropositive individuals (71%). The correlation between OD obtained with ELISA and antibody titre obtained with MLPA was statistically significant (r = 0.70, p less than 0.001). Conversely to ELISA, MLPA was not applicable on blood samples absorbed on filter paper without a serious loss of sensitivity. In conclusion, this study demonstrated that the MLPA test can only reliably detect anti-PGLI antibodies in multibacillary cases.
- Published
- 1991
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