1. Enhanced microfiltration devices configured with hydrodynamic trapping and a rain drop bypass filtering architecture for microbial cells detection
- Author
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Liang Zhu, Christophe Lay, Ramana Murthy, Wen Tso Liu, Cheng Yong Teo, H.H. Feng, Bi Rong Chew, Xue Li Peh, and Hong Miao Ji
- Subjects
Materials science ,Microfiltration ,Fluoroimmunoassay ,Biomedical Engineering ,Bioengineering ,Hydrodynamic trapping ,Biochemistry ,Sensitivity and Specificity ,Fluorescence ,parasitic diseases ,Escherichia coli ,Animals ,Fluorescent Antibody Technique, Indirect ,Cryptosporidium parvum ,Previous generation ,Chromatography ,business.industry ,Drop (liquid) ,General Chemistry ,Filter (signal processing) ,Equipment Design ,Microfluidic Analytical Techniques ,Cell loss ,Lower pressure ,Optoelectronics ,Rain drop ,Giardia lamblia ,business - Abstract
Ultra-fine (1 microm) microfilters are required to effectively trap microbial cells. We designed microfilters featuring a rain drop bypass architecture, which significantly reduces the likelihood of clogging at the cost of limited cell loss. The new rain drop bypass architecture configuration has a substantially lower pressure drop and allows a better efficiency in trapping protozoan cells (Cryptosporidium parvum and Giardia lamblia) in comparison to our previous generation of a microfilter device. A modified version displaying sub-micron filter gaps was adapted to trap and detect bacterial cells (Escherichia coli), through a method of cells labeling, which aims to amplify the fluorescence signal emission and therefore the sensitivity of detection.
- Published
- 2008