1. A cost-effective fluorescence mini-microscope for biomedical applications
- Author
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Zhang, Yu Shrike, Ribas, João, Nadhman, Akhtar, Aleman, Julio, Selimović, Šeila, Lesher-Perez, Sasha Cai, Wang, Ting, Manoharan, Vijayan, Shin, Su-Ryon, Damilano, Alessia, Annabi, Nasim, Dokmeci, Mehmet Remzi, Takayama, Shuichi, and Khademhosseini, Ali
- Subjects
Biotechnology ,Bioengineering ,Animals ,Cell Movement ,Fluorescent Dyes ,Hep G2 Cells ,Humans ,Lab-On-A-Chip Devices ,Mice ,Microscopy ,Fluorescence ,NIH 3T3 Cells ,Oxygen ,Chemical Sciences ,Engineering ,Analytical Chemistry - Abstract
We have designed and fabricated a miniature microscope from off-the-shelf components and a webcam, with built-in fluorescence capability for biomedical applications. The mini-microscope was able to detect both biochemical parameters, such as cell/tissue viability (e.g. live/dead assay), and biophysical properties of the microenvironment such as oxygen levels in microfabricated tissues based on an oxygen-sensitive fluorescent dye. This mini-microscope has adjustable magnifications from 8-60×, achieves a resolution as high as
- Published
- 2015