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거대쌀눈의 특성과 검정쌀 그리고 찹쌀의 특성을 보유한 눈큰흑찰의 침종과 발아 그리고 기질로 글루탐산을 처리하였을 경우의 가바를 포함한 주요 성분의 분석 결과는 다음과 같다. 1. 싹 발아 길이를 기준으로 눈큰흑찰의 가바 함량은 싹 길이가 5~10 mm인 stage-3에 93.9 mg/100 g으로 최고를 보였고, 대량 제조 조건에서는 최고 126.9 mg의 가바를 함유하고 있음을 확인하였다. 2. 침종에 의한 성분 분석에서는 72시간 침종에서 폴리페놀, 플라보노이드, 아미노산 그리고 가바 함량이 가장 많이 축적됨을 확인하였다. 3. 글루탐산 탈탄산효소의 작용에 의한 가바 함량 증가는 현미와 쌀겨 모두에서 급격한 증가가 관찰되었고, 눈큰흑찰 현미와 쌀겨의 경우 가바 함량이 각각 354.6 mg/100 g과 726.4 mg으로 현미와 쌀겨에 비해 각각 14배와 3배 함량이 증가하였다. 따라서 본 연구에서 눈큰흑찰의 발아와 침종에 따른 품질특성을 분석한 결과, 뇌의 대사전달 물질의 조정 작용과 고혈압 그리고 스트레스 조정 기능이 있는 가바의 함량이 침종과 발아에 의해 일반 쌀에 비해 급격히 증가함을 확인하였다. 특히나 글루탐산탈탄산 효소의 작용에 의한 눈큰흑찰의 현미와 쌀겨의 가바 생성은 현재까지 보고된 그 어떤 쌀품종에서 분석된 가바 함량보다도 가장 우수함을 확인할 수 있어 향후 기능성 발아현미 가공을 위한 원료로 활용이 가능하며, 침종 조건은 가바 함량이 증진된 밥의 제조에 활용이 가능하고, 일본에서 시판되는 가바 건강식품의 용량과 맞먹는 함량을 지닌 눈큰흑찰을 이용한 가바쌀 생산과 가바의 효능에 의한 혈압강하, 체중조절, 알코올 관련 질환 등에 효능이 있는 건강기능성 식품 원료로 활용이 가능할 것으로 사료된다. 【We assessed the GABA accumulation and other components after the 'Nunkeunhukchal (BGE)', 'Josanghukchal (BR)', and 'Ilmibyeo (IB)' grain was soaked in water for 24, 36, 48, 60, 72 and 96 hr. The results showed a continuous accumulation of GABA in soaking treated brown rice of BGE and IB. Among the treated hours, 72 hours of soaking had the maximal accumulation of GABA (51.4 mg/100 g), amino acid, polyphenol and other components. The activities of glutamate decarboxylase (GAD) in brown rice and rice-bran were the same in BGE rice. However, the formation of GABA treated with L-glutamate as substrate showed dramatic increase of 354.6 (fourteen times higher than normal extraction) and 726.4 mg/100 g in BGE rice and rice-bran, respectively. These results suggested that the soaking and extraction with L-glutamate buffer could be better methods for the harvest of increased GABA.】

Sorghum has been consumed as one of the important staple food in the semiarid tropics of Africa and Asia. Sorghum is rich in starch, protein, essential vitamins and minerals and grows relatively well in dry climate regions when it compared with other staple food crops. Sorghum has taken an increased interest due to several studies that report about the beneficial effects of sorghum on human health. In the present study, we investigated the antioxidative and activity of extract of milling by-products (hull and bran) of Korean sorghum cultivar, `Hwanggeaumchal` as well as its grain. Hull extract showed the highest total polyphenol contents () and major four pigments content (). From results of 2,2`-azinobis-(3-ethylbenzothiazoline-6-sulfonate (ABTS) radical scavenging activity, hull extract (, ) was also showed the strongest antioxidative effects. Bran and grain showed similar polyphenol, pigments contents and antioxidative effects. We determined cell viability by MTT assay and evaluated the anti-inflammatory activity by measuring nitric oxide (NO) of hull, bran and grain methanol extract (0.5% HCl v/v) on RAW 264.7 cells. Hull extract treatment was significantly decreased NO production with dose-dependant manner. Apigeninidin as one of the major pigment of hull was showed inhibitory activity against NO production without cytotoxicitiy. Therefore, sorghum milling by-products can be used as a good source of antioxidative and anti-inflammatory agents.

Red yeast rice (RYR) is the product of fermented yeast by Monascus strains on rice, and has recently become a popular dietary supplement as a traditional food in Asia. RYR contains monacolin K substances known to inhibit cholesterol synthesis as efficiently as statin drugs. To determine the optimal rice cultivar for manufacturing RYR, 7 rice cultivars (Goami, Goami2, Sangjuchalbyeo, Seolgaeng, Saegyejinmi, Yeonghojinmi and Chilbo) were fermented using two Monascus strains ( M. ruber KCTC6122 and KCCM60141 of M. ruber ) in this study. The monacolin K content of Sangjuchalbyeo were 47.24 ppm on KCTC6122 cultures and 117.03 ppm on KCCM60141 cultures, respectively. Other cultivars, especially Goami and Goami2, which had less content of monacolin K could not seem to ferment normally because those didn’t show red color. These results imply that Sangjuchalbyeo can be optimal rice cultivar as a commercial RYR which is well fermented rice and has high content of monacolin K. Keywords

The main challenges for the development of agricultural bio-degradable mulching film concern the degradation during the lifetime of cultivated crops. A set of rice by-product (rice-hull and rice-bran) based bio-degradable mulching films was developed and tested, following the measurement of standard bio-degradability rate and adaptability in a large scale field experiment. The standard bio-degradability of bio-film passed the KS (Korea standard) regulation. The result of mechanical analysis of bio-degradable mulching film presented a higher mechanical strength and elongation rate compared with polyethylene film. In addition, bio-film could be degraded into fragments within 4 months under the field condition of several upland crops. Bio-degradable mulching film indicated great potential as a new source of agricultural bio-degradable material.

Seed germination and dormancy of three green manure seeds such as hairy vetch, crimson clover and narrow-leaved vetch (Vicia angustifolia L.) produced in Milyang, southern part of Korea were investigated to provide a basic information on the stability of seedling establishment in green manure seeds. In addition. the effect of seed storage duration on the germinability of imported hairy vetch and crimson seeds was also investigated. To determine the seed maturity time of three green manure seeds, the seeds were harvested manually at May 25, May 30, and June 4, and the 100-seed weight, germinability and percentage of hard seed were evaluated. Freshly harvested seeds of three green manure crops were strongly dormant because of hard seed coat. Germination percentage of the three green manure crops varied depending on the seed harvest time ranging from 30 - 52% in hairy vetch, 16 - 35% in crimson clover and 2 - 61% in V. angustifolia, respectively. Of the three crops, crimson clover and V. angustifolia matured early on May 30 to June 4 (around 35 to 40 days after flower) but hairy vetch seed did not mature until June 4 based on the seed germinability. The matured seed of crimson clover and V. angustifolia germinated less than 20% while scarification on seed coat significantly overcome the innate dormancy. On the other hand, the seed germination of hairy vetch and crimson clover was lower under dark than in the presence of light. The germination percentage of the imported hairy vetch and crimson clover seeds showed high with 77 - 79%, 94 - 95%, respectively, but the seed germination significantly declined by 21 - 32%, 30 - 40% after one-year of storage under natural conditions. The seeds germinated only 8 - 13% for hairy vetch but crimson clover did not germinate at all after two years storage. These results indicate V. angustifolia and crimson clover mature at May 30 to June 4 but hairy vetch did not. The imported hairy vetch and crimson clover seeds should be used within one-year after import for stable seedling establishment in green manure-rice cultivation cropping system.

This study was aimed to develop blend films by rice by-product (rice-hull and rice-bran) and bio-degradable materials. The rice by-product was firstly prepared from the pulverizing for making fine powder. Bio-degradable materials could be prepared by melting at high temperature. The mixture of the fine powder of rice by-product and melted bio-degradable materials was then blended and cast into films. The obtained films were investigated on their morphology, secondary structures and properties by using SEM, ICP and ASTM, respectively. Mechanical properties and degradability of these films were measured and compared to those of the PE films. Mechanical strength of bio-films was higher than that of PE films, however elongation ratio showed lower percent than that of PE film. In addition, bio-film could be degraded into fragments within 3 months under the field condition of normal upland crop cultivation. Bio-degradable mulching film indicated great potential for agronomic use as a new source of bio-degradable material.

The bio-degradable transplanting pot containing rice by-product (rice-hull and rice-bran) were developed, and tested their ability for agronomic use. Rice by-products were crosslinked with biodegradable aliphatic ally aromatic copolyesters or urea resin for making transplanting pot. Mechanical properties and degradability of these pots were measured and compared to those of the Jiffy pot (commercially used bio-degradable pot). Mechanical strength was higher than that of Jippy pot, and bio-degradability was excellent under the actual field condition. In addition, the pot could be degraded within 3 months under the ground. Our result indicated bio-degradable pot containing rice by-products has a great potential for such agronomic use.