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Investigating beneficial chemical compounds of plant extracts is one of the ways to prevent biodiversity loss. This study evaluated the antimicrobial activity of indigenous plant extracts from Toba , North Sumatra, and Mt. Merapi National Park regions against Escherichia coli , Bacillus subtilis , Salmone l la typ h i , Staphylococcus aureus , Candida albicans , and Candida tropicalis by calculating the zone of microbial growth inhibition. Among the plant extracts, T 5 that identified as Toona sinensis showed the highest microbial inhibit ion to the growth of C. albicans , B. subtilis , S. typhi, and E. coli with the diameter growth of approximately 2.00, 1.80, 1.33, and 1.33 cm, respectively. Based on those results, T. sinensis was then subsequently fractionated using n-hexane, ethyl acetate, and methanol , respectively. The resulted fractions also were evaluated for antimicrobial bioassay. A ll fractions have shown activity in inhibiting the growth of the microbes at 1% concentration. However, each fraction show ed growth inhibition against certain microbe s . The n-hexane fraction show ed the greatest inhibitory activity for E. coli and S. typhi ; ethyl acetate fraction for B. subtilis and C. albicans ; and methanol fraction for E. coli and S. typhi . The results revealed that T. sinensis plant extract has great potential as an antimicrobial agent . Further investigation is needed to observe the mode of anti microbial action of fractionated crude extracts of T . Sinensis . The exploring potency of Indonesian biodiversity opened up a new way for the utilization of plants for economic development and conservation . Keywords: antimicrobial plants, indigenous knowledge, Toba-North Sumatra, Mt. Merapi