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Pennahia anea is among of the demersal fish landed at Asemdoyong Fish Auction Centre, Pemalang. P. anea doesn't show sexual dimorphism, so that other characters are needed to differentiate male and female individuals, i.e. truss morphometric and meristic characters. Previous studies have shown that truss morphometric and meristic can differentiate between male and female individuals. This study aims to describe the morphometric and meristic truss characters and determine the truss morphometrically and meristic characters that distinguish between males and females of Pennahia anea. The research used a survey method with a purposive random sampling technique. Male and female individuals of P. anea were examined based on their meristic and truss morphometric characters. The data were analyzed statistically using Mann Whitney non-parametric test. The result proved that male and female were significantly different in seven out of the 31 truss distances. Male individuals had a larger size than female individuals in three truss distances. In contrast, the male had a smaller size than the female individuals in four out of seven truss distances that distinguish both sexualities. The soft fin radius of the ventral fin was significantly different between male and female of P. anea. It could be concluded that male and female individuals of Pennahia anea have different truss distances and meristic characters with seven truss distances and one meristic character are different

Segara Anakan areas can be divided into three different regions according to their salinity. Salinity differences suggested that Commerson’s anchovy population in that area can be divided into three subpopulations due to genetic differences. Genetic differences among subpopulation can be assessed through a population genetic study using random amplified polymorphic DNA. This study aims to evaluate the genetic variation and differences of Commerson's anchovy (Stolephorus commersonnii) collected at three different water salinities in Segara Anakan estuary Cilacap Indonesia. Total genomic DNA was isolated using the Chelex method. Genetic diversity and differences were assessed using RAPD markers and were analyzed statistically using an analysis of molecular variance, as implemented in Arlequin software. The results showed that high genetic diversity was observed within the subpopulations. However, no significant genetic differences were observed among subpopulations which indicate genetic similarity. A high number of offspring are likely to cause high genetic variation within subpopulations. Adult and larvae migration is the cause of genetics similarity across Segara Anakan. Another impressive result is that water salinity did not affect the genetic characteristic of Commerson,s anchovy. Genetic similarity of Commerson’s anchovy indicates that Segara Anakan forms a single genetic conservation unit.