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ABSTRAK. Penelitian ini bertujuan mengetahui pengaruh pemberian hormon PGF2α sebelum koleksi terhadap peningkatan kualitas semen dan level testosteron sapi Aceh di UPT. Hewan Coba Universitas Syiah Kuala. Penelitian ini dilaksanakan dari bulan Januari sampai dengan Maret 2019. Data hasil pemeriksaan kualitas semen dianalisis secara deskriptif. Dalam penelitian ini digunakan satu ekor sapi Aceh jantan sebagai kontrol dengan 2 perlakuan, yaitu P1 (tanpa pemberian PGF2α sebelum koleksi) dan P2 (pemberian PGF2α 30 menit sebelum koleksi) dengan dosis 25 mg tiap perlakuan. Setiap perlakuan dilakukan sebanyak lima kali dan dilakukan secara bergantian setiap minggunya. Rata-rata volume (ml); konsentrasi (106 sel/ml); motilitas (%), viabilitas (%); abnormalitas (%); dan level testosteron (ng/ml) pada P1 vs P2 masing-masing adalah 5,2±1,30 vs 5,2±0,97 (P0,05); 145,4±36,58 vs 172,8 ±46,27 (P0,05); 63,87±6,44 vs 75,05±0,08 (P0,05); 69,30±0,22 vs 75,05±0,08 (P0,05); 16,90±0,08 vs 10,46±0,09 (P0,05); dan 28,07±14,37 vs 24,19±6,11 (P0,05). Disimpulkan bahwa pemberian PGF2α 30 menit sebelum koleksi dapat meningkatkan kualitas spermatozoa tetapi tidak dapat meningkatkan konsentrasi testosteron. (Case study the effect of giving pgf2α before the collection of the quality of Aceh cattle semen) ABSTRACT. This study aims to determine the effect of giving PGF2α hormone before collection to improve the quality of Acehnese semen in UPT. Hewan Coba Syiah Kuala University. This research was conducted from January to March 2019. Data on semen quality examination were analyzed descriptively. In this study, one Aceh cattle was used as a control with 2 treatments, namely P1 (without administration of PGF2α before collection) and P2 (administration of PGF2α 30 minutes before collection) at a dose of 25 mg per treatment. Each treatment was carried out five times and carried out alternately every week. The results showed that the average volume of cement (ml) and pH P1 and P2 respectively 5.2 ± 1.30 and 5.2 ± 0.97, and 6.6±0.55 and 6.5 ± 0.50. While the average concentration of spermatozoa (106 cells / ml) in P1 and P2 were 145.4 ± 36.58 and 172.8 ± 46.27 respectively. The average percentage (%) of progressive spermatozoa motility, circularity, non motility, viability, and abnormalities in P1 and P2 respectively: 63.87 ± 6.44 and 73.63 ± 9.42, 11.34 ± 3, 70 and 9.0 ± 7.01, 24.79 ± 4.04 and 17.37 ± 4.44, 69.30 ± 0.22 and 75.05 ± 0.08, 16.90 ± 0.08 and 10.46 ± 0.09. It was concluded that administration of PGF2α 30 minutes before collection can increase the quality of spermatozoa but cannot increase the concentration of testosterone.

ABSTRAK. Penggunaan human cortisol enzyme-linked immunosorbent assay (ELISA) kit untuk pengukuran hormon kortisol pada hewan dan keterlambatan waktu sentrifugasi sampel darah untuk analisis hormon perlu dievaluasi. Penelitian ini bertujuan untuk mengevaluasi validitas human cortisol ELISA kit (EIA-1887, DRG Instruments GmbH, Germany) untuk pengukuran konsentrasi kortisol dan menguji pengaruh keterlambatan waktu sentrifugasi sampel darah terhadap stabilitas konsentrasi kortisol pada kambing kacang. Sampel darah dikoleksi dari delapan ekor kambing kacang. Uji validitas kit EIA-1887 dilakukan secara: a) analitik (uji parallelism, akurasi, dan presisi), dan b) biologis (pengukuran kortisol sebelum dan setelah transportasi). Uji keterlambatan waktu sentrifugasi terhadap stabilitas konsentrasi kortisol dilakukan dengan 5 perlakuan yaitu disentrifugasi kurang dari 1 jam (P1/kontrol), 6 jam (P6), 12 jam (P12), 18 jam (P18),dan 24 jam (P24) setelah darah dikoleksi. Data uji parallelism dianalisis dengan uji persamaan kemiringan, uji presisi dihitung % CV (coefficient variation) intra-assay dan inter-assay, uji akurasi dihitung % recovery, uji T untuk validasi biologis, dan uji ragam (One Way Anova) untuk pengaruh waktu sentrifugasi. Hasil uji parallelism menunjukkan kurva sampel kambing kacang sejajar/parallel dengan kurva standar kortisol. Akurasi kit EIA-1887 adalah 103,43±7,85%, dan % CV intra-assay dan inter-assay adalah 10%. Konsentrasi kortisol setelah transportasi secara signifikan lebih tinggi daripada sebelum transportasi (p0,05). Adanya penurunan secara nyata konsentrasi kortisol pada darah yang disentrifugasi 24 jam (P24) setelah koleksi (p0,05). Kesimpulan, human cortisol ELISA kit (EIA-1887) memiliki validitas yang baik secara analitik dan biologis untuk digunakan dalam pengukuran konsentrasi kortisol pada kambing kacang. Keterlambatan waktu sentrifugasi selama 24 jam berpengaruh terhadap konsentrasi kortisol. (Evaluation the Validity of Human Cortisol Enzyme-Linked Immunosorbent Assay (ELISA) Kit and Centrifugation Time of Blood Sample for Measuring the Concentration of Cortisol in Kacang Goats) ABSTRACT. The use of human cortisol ELISA kit for measuring cortisol in animals and delayed to blood centrifugation time for hormone measurement need to be evaluated. This study aimed to evaluate the validity of human cortisol ELISA kit (EIA-1887, DRG Instruments GmbH, Germany) for cortisol measurement and effect of delayed to blood centrifugation time on cortisol concentrations in kacang goats. Blood was collected from eight kacang goats. Validation test of EIA-1887 kit was performed through: a) analytical (parallelism, accuracy, and precision tests), and b) biological validations (measuring cortisol concentrations before and after transportation). Five treatments were performed to test delayed to centrifugation time: blood centrifuged at 1 h (control, P1), 6 h (P6), 12 h (P12), 18 h (P18), and 24 h (P24) after collection. Parallelism data were analyzed by slope equality test, precision and accuracy calculated by % CV of intra-and inter-assay, and % recovery, respectively. Data of biological validation and centrifugation time effects were analyzed by Student t-test, and one way ANOVA, respectively. Results of parallelism showed that serial dilution curve of kacang goat plasma was parallel with cortisol standard curves. Accuracy of EIA-1887 kit was 103.43±7.85%, and % CV of intra-and inter-assay were 10%. Concentration of cortisol after transportation was significantly higher than before transportation (p0.05). Concentration of cortisol was significantly decreased when blood was centrifuged at 24 h after collection (P0.05). In conclusion, human cortisol ELISA kit (EIA-1887) is a reliable assay for measuring cortisol in plasma of kacang goat. Delayed to blood centrifugation time affect cortisol concentrations.

ABSTRAK. Penelitian ini bertujuan mengetahui pengaruh perbedaan PGF2α analog terhadap persentase berahi sapi di Labuhanbatu Selatan. Sampel yang digunakan dalam penelitian ini adalah data hasil UPSUS SIWAB tahun 2017. Dalam penelitian ini digunakan data 2.547 ekor sapi yang memiliki variasi breed dan umur, dengan skor kondisi tubuh baik. Sapi-sapi dikelompokkan menjadi tiga kelompok berdasarkan preparat sinkronisasi berahi yang digunakan. K1= kelompok sapi yang diinjeksi dengan 5 ml PGF2α-1 (dinoprost tromethamine 5 mg/ml dan benzil alkohol 1,65%) berjumlah 1.300 ekor. K2= kelompok sapi yang diinjeksi dengan 5 ml PGF2α-2 (dinoprost tromethamine 5,5 mg/ml dan benzil alkohol 12,0 mg/ml) berjumlah 600 ekor. K3= kelompok sapi yang diinjeksi dengan 2 ml PGF2α-3 (cloprostenol 75 mg/ml dan chlorocresol 1,0 mg/ml) berjumlah 647 ekor. Penyuntikan dilakukan secara intramuskulus, dua kali dengan interval 10 hari. Data yang diperoleh kemudian dianalisis menggunakan uji Kruskal Wallis dan dilanjutkan dengan uji Mann Withney. Hasil analisis menunjukkan persentase berahi pada kelompok 1; 2; dan 3 masing-masing 80,7%; 50% dan 61,8% (P0,01). Dari hasil penelitian dapat disimpulkan bahwa PGF2α-1 mempunyai efektivitas lebih baik dibandingkan PGF2α-2 dan PGF2α-3 dalam induksi sinkronisasi berahi pada sapi di Kabupaten Labuhanbatu Selatan, Sumatera Utara. (Effectiveness of different commercial prostaglandin hormone administration on the percentage of estrous in cattles in South Labuhanbatu, North Sumatera) ABSTRACT. The aim of this study was to determine the effect of differences PGF2α analogues on the percentage of estrus in cattles in South Labuhanbatu. The sample used in this study was the 2017 SIWAB UPSUS data. Data of 2,547 cattles with various breed and age as well as in good body condition scores were selected. The cattles were grouped into three data groups based on the hormone used for estrus synchronization. The cattles in data group I (1300 cows) were injected with 5 ml PGF2α-1 (dinoprost tromethamine 5 mg/ml and benzyl alcohol 1.65%). The cattles in data group II (600 cows) were injected with 5 ml PGF2α-2 (5.5 mg/ml dinoprost tromethamine and 12.0 mg/ml benzyl alcohol). The cattles in data group III (647) were injected with 2 ml of PGF2α-3 (cloprostenol 75 µg/ml and chlorocresol 1.0 mg/ml). Injections were carried out intramuscularly, twice at 10-day intervals. The data obtained were then analyzed Kruskal Wallis and continued with Mann Withney test. The results showed that the percentage of estrus in groups I; II; and III were 80.7%; 50% and 61.8% (P0.01). From the results of the study it can be concluded that Lutalyse has better effectiveness than Capriglandin and Sincrovall in the induction of synchronization estrus in cattle in South Labuhanbatu, North Sumatera.