Search

研究MoYpt7 与MoGdi1 的互作关系以及定点突 变体MoYpt7N125I的生物学功能。 补充了MoYpt7 在稻瘟病菌致病过程中的生物学 功能。 采用双分子荧光互补和免疫共沉淀的方法检测 MoYpt7 与MoGdi1 的相互作用。利用点突变的方 法,分析MoYpt7 的鸟嘌呤二核苷酸磷酸(GDP) 结合形式的生物学功能。 MoYpt7 与Rab-GDP 解离抑制因子的一个同源物 MoGdi1 相互作用,获得了MoYpt7 的GDP 结合 形式的过表达突变体。该突变体菌落形态发生变 化,产孢能力和致病性显著下降。同时,稻瘟病 菌MoYpt7N125I 的亚细胞定位发生变化。

Alkaloids are plant secondary metabolites that are widely distributed in Nicotianaspecies and contribute greatly to the quality of tobacco leaves. Some alkaloids, such as nornicotine and myosmine, have adverse effects on human health. To reduce the content of harmful alkaloids in tobacco leaves through conventional breeding, a genetic study of the alkaloid variation among different genotypes is required. In this study, alkaloid profiles in leaves of five Nicotiana tabacumcultivars and Nicotiana tomentosiformiswere investigated. Six alkaloids were identified from all six genotypes via gas chromatograph-mass spectrometry (GC-MS). Significant differences in alkaloid content were observed both among different leaf positions and among cultivars. The contents of nornicotine and myosmine were positively and significantly correlated (R2=0.881), and were also separated from those of other alkaloids by clustering. Thus, the genotype plays a major role in alkaloid accumulation, indicating a high potential for manipulation of alkaloid content through traditional breeding.

A mature appressorium cDNA library of rice blast fungus, Magnaporthe griseawas constructed in a λ TriplEx2 vector by SMART™cDNA library containing 2.37×106independent clones about 100% of which harbor foreign cDNA inserts with average size of 660 bp. Of 9 randomly selected clones, 2 expressed sequence tags (ESTs) sequences did not have homologous EST sequences of M. griseain GenBank. The appressorium cDNA library is suitable for gene expression analysis and function analysis of the late stages of appressorium formation and the early stages of penetration of M. grisea.