1. Comparison of antimicrobial activity of selected, commercially available wound dressing materials.
- Author
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Szweda, Piotr, Gorczyca, Grzegorz, and Tylingo, Robert
- Subjects
COMPARATIVE studies ,ESCHERICHIA coli diseases ,GRAM-negative bacterial diseases ,PSEUDOMONAS diseases ,RESEARCH funding ,SURGICAL dressings ,WOUND care ,TREATMENT effectiveness ,DATA analysis software ,DESCRIPTIVE statistics - Abstract
Objective: The aim of our study was to examine the antimicrobial potential of eight selected, commercially available wound dressings containing different antimicrobial agents: silver, chlorhexidine acetate, povidone-iodine, and manuka honey. Method: The materials were tested against four reference strains of bacteria:
Staphylococcus aureus (PCM 2051),Staphylococcus epidermidis (PCM 2118),Pseudomonas aeruginosa (ATCC 27853), andEscherichia coli (K12), using the disc diffusion-like method and a time-killing assay. Results: For both experiments, the highest activity against all four tested strains of bacteria was observed in the case of Mepilex Ag, which contains silver as an antibacterial agent. Incubation for four hours of a 10x10mm2 piece of this material in 10ml cells suspension (concentration: 109 –1010 CFU/ml) resulted in complete elimination of bacteria of all four strains tested. The same results were obtained for a povidone-iodine containing dressing, Inadine, though its activity was lower in the disc diffusion assay. Silvercel, Aquacel Ag and Melgisorb Ag, which also contain silver, also exhibited a satisfactory level of activity. In the case of Aquacel Ag, 24 hours' incubation resulted in complete elimination of the cells of both Gram-negative bacteria,Escherichia coli andPseudomonas aeruginosa .TheEscherichia coli cells were killed after only four hours' treatment. High effectiveness againstEscherichia coli was also demonstrated for Silvercel. However, 24 hours' includation was required for complete elimination of the cells of this bacteria strain. High activity against all tested bacteria, but only in the disc diffusion assay, was observed for Algivon, which contains manuka honey. The Medisorb Silver Pad, containing silver, and Bactigras, which contains chlorhexidine acetate, revealed much lower antimicrobial activity, particularly noticeable in the time-killing assay. In addition, we also tested the anti-staphylococcal activity of a biopolymer material impregnated with lysostaphin. Results revealed that its activity againstStaphylococcus aureus was comparable to the most active wound dressings impregnated with silver or inadine. Conclusion: Some important differences in the antimicrobial potential of investigated materials have been found. The presented results could be of interest to clinicians managing wounds. [ABSTRACT FROM AUTHOR]- Published
- 2018
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