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Two experiments were conducted to study Gloiopeltis furcata spore culture with different substrates in the nonreproduction season. Using spores discharged by fertile G. furcata frozen for storage, two experiments were conducted during the nonreproduction season. In the first experiment, G. furcata spores were collected and incubated in aquariums using palm line, polythene line, vinylon line, and three types of plastic sheets (white, ripple, and transparent polymethyl methacrylate) as substrates. In the second experiment, G. furcata spores were collected and incubated in a plastic tank using plastic sheets of different materials (PP, PE, and transparent PVC) and plastic even nets of different mesh sizes (3, 2, and 0.5 cm) as substrates. At the end of the experiment (after 60 d), discs with raised thallus were found on different plastic sheets and white plastic even nets, but no discs were visible on any type of line or on the black plastic even net (3-cm mesh). The white plastic even net was found to be a suitable substrate for G. furcata spore culture in farms and for sporeling culture in sea. Results presented in this study are expected to aid in the progress of the artificial seeding of Gloiopeltis.