1. Metabolomics-Based Discovery of Biomarkers with Cytotoxic Potential in Extracts of Myracrodruon urundeuva
- Author
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Licia dos Reis Luz, Edy Sousa de Brito, Jhonyson Arruda Carvalho Guedes, Paulo Riceli Vasconcelos Ribeiro, Maria Francilene Souza Silva, Guilherme Julião Zocolo, Caio B. Castro, Cláudia Pessoa, Gisele Silvestre da Silva, Davila Zampieri, Kirley Marques Canuto, Diogo Denardi Porto, CAIO BEZERRA DE CASTRO, Universidade Federal de São Carlos. Departamento de Química, EDY SOUSA DE BRITO, CNPAT, DÁVILA DE SOUZA ZAMPIERI, Universidade Federal do Ceará. Departamento de Química Orgânica e Inorgânica., ClLAUDIA DO Ó PESSOA, Universidade Federal do Ceará. Núcleo de Pesquisa e Desenvolvimento de Medicamentos (NPDM)., GUILHERME JULIAO ZOCOLO, CNPAT., LICIA DOS REIS LUZ, Universidade Federal do Ceará. Departamento de Química Analítica e Físico-Química., JHONYSON ARRUDA CARVALHO GUEDES, CNPAT, DIOGO DENARDI PORTO, CPATSA, MARIA FRANCILENE SOUZA SILVA, Universidade Federal do Ceará. Núcleo de Pesquisa e Desenvolvimento de Medicamentos (NPDM), GISELE SILVESTRE DA SILVA, CNPAT, PAULO RICELI VASCONCELOS RIBEIRO, CNPAT, and KIRLEY MARQUES CANUTO, CNPAT
- Subjects
Quimiometria ,Aroeira do sertão ,Vegetação Nativa ,Electrospray ionization ,Cytotoxicity ,Atividade citotóxica ,Mass spectrometry ,Myracrodruon Urundeuva ,Caatinga ,chemistry.chemical_compound ,Metabolomics ,Espécie Nativa ,Bioma Caatinga ,Phenols ,Chemometrics ,Myracrodruon urundeuva ,Chromatography ,biology ,General Chemistry ,biology.organism_classification ,Anacardic acids ,Aroeira ,chemistry ,Phytochemical ,UPLC-MS/MS ,visual_art ,visual_art.visual_art_medium ,Bark - Abstract
Myracrodruon urundeuva (aroeira-do-sertão) is a species threatened with extinction due to anthropogenic exploitation. Phytochemical analysis of bark, branch and leaf extracts revealed the presence of several compounds such as flavonoids, phenols, tannins, quercetin derivatives and anacardic acids. Dereplication methodology was performed to tentatively identify 50 compounds analyzed by ultra-performance liquid chromatography coupled with an electrospray ionization quadrupole time-of-flight mass spectrometry operating in MSE mode (UPLC-QTOF-MSE). The extracts exhibited anti-tumor effect in cancer cells HCT-116 (colorectal), SF-295 (glioblastoma), HL-60 (leukemia), and RAJI (leukemia). Also, these results correlate with the principal component analysis (PCA) data that identified three distinct groups indicating, efficiently, metabolic differences between organs of M. urundeuva. Through discriminatory analysis of the orthogonal partial least squares (OPLS-DA), the variable of importance in the projection (VIP) and S-Plot, we were able to determine 30 potential biomarkers. The fingerprint of hydroethanolic extracts was correlated with the cytotoxicity assay and demonstrated a significant difference in the composition of plant extract. Made available in DSpace on 2020-01-24T18:17:42Z (GMT). No. of bitstreams: 1 MetabolomicsBasedDiscoveryofBiomarkerswith2019.pdf: 3991156 bytes, checksum: 1d6d7fbdb69123f422ac2fdeac45d5cb (MD5) Previous issue date: 2019 On-line
- Published
- 2020
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