1. Isolation, Absolute Configuration and Cytotoxic Activities of Alkaloids from Hippeastrum goianum (Ravenna) Meerow (Amaryllidaceae)
- Author
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Andrea N. L. Batista, Letícia V. Costa-Lotufo, Mariacaterina Lianza, Warley de Souza Borges, Jaume Bastida, Maria Helena Verdan, Álvaro Cunha Neto, João M. Batista, Sarah Christina Caldas Oliveira, Larissa Costa de Almeida, Jean Paulo de Andrade, Ferruccio Poli, Mariacaterina Lianza, Maria Helena Verdan, Jean Paulo de Andrade, Ferruccio Poli, Larissa de Almeida, Leticia Costa-Lotufo, Álvaro Cunha Neto, Sarah Oliveira, Jaume Bastida, Andrea Batista, João Batista Jr., and Warley Borges
- Subjects
Stereochemistry ,Circular dichroism ,FARMACOLOGIA ,Cell-mediated cytotoxicity ,narciclasine, galasine, cytotoxic activity, absolute configuration, vibrational, circular dichroism ,Alkaloids ,Hippeastrum ,Alcaloides ,Dicroisme circular ,Cytotoxicity ,IC50 ,cytotoxic activity ,Tumors ,biology ,Chemistry ,Absolute configuration ,General Chemistry ,Amaryllidaceae ,vibrational circular dichroism ,biology.organism_classification ,Citotoxicitat per mediació cel·lular ,absolute configuration ,Phytochemical ,Cell culture ,Vibrational circular dichroism ,galasine ,narciclasine - Abstract
The phytochemical study of Hippeastrum goianum led to the identification of 13 compounds by means of gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR). Compounds 7-demethoxy-9-O-methylhostasine (1) and 7-deoxi-trans-dihydronarciclasine (2) had their absolute configurations determined by vibrational circular dichroism (VCD). This is the first time that compound 1 is described in the Amaryllidaceae family. The cytotoxicity of all isolated compounds was tested against colorectal carcinoma (HCT 116), breast carcinoma (MCF-7), and non-tumor human retinal pigment epithelium (RPE) cell lines. The half-maximum inhibitory concentration (IC50) of compound 2 against each cell line was equivalent to the positive control (doxorubicin), indicating a considerable cytotoxic activity. Keywords: narciclasine; galasine; cytotoxic activity; absolute configuration; vibrational circular dichroism
- Published
- 2020
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