1. Enantiomeric Recognition of D- and L‑Lactate by CEST with the Aid of a Paramagnetic Shift Reagent.
- Author
-
Lei Zhang, Martins, André F., Piyu Zhao, Tieu, Michael, Esteban-Gómez, David, McCandless, Gregory T., Platas-Iglesias, Carlos, and Sherry, A. Dean
- Subjects
- *
LACTATES , *NUCLEAR magnetic resonance , *CANCER cell analysis , *STEREOSELECTIVE reactions , *METABOLITES , *DENSITY functional theory - Abstract
A previous report demonstrated that EuDO3A could be used as an NMR shift reagent for imaging extracellular lactate produced by cancer cells using CEST imaging. In this work, a series of heptadentate macrocyclic YbDO3A-trisamide complexes with δ-chiral carbons in the three pendant side-arms were examined as shift reagents for lactate detection. High resolution ¹H NMR spectra and DFT calculations provided evidence for the formation of stereoselective lactate·YbDO3Atrisamide complexes each with a different CEST signature. This stereoselectivity allowed discrimination of D- versus L-lactate by both high-resolution NMR and CEST. This work demonstrates that lanthanide-based paramagnetic shift reagents can be designed to detect important metabolites by CEST MRI selectively. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF