1. Selective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore
- Author
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Susan Chalmers, Andrew M. James, Michael P. Murphy, John G. McCarron, Richard C. Hartley, Tracy A. Prime, Stuart T. Caldwell, Andrew G. Cairns, and Caroline Quin
- Subjects
Protonophore ,Ultraviolet Rays ,Cell ,Myocytes, Smooth Muscle ,Mitochondrion ,Matrix (biology) ,01 natural sciences ,Biochemistry ,Catalysis ,03 medical and health sciences ,Colloid and Surface Chemistry ,Drug Delivery Systems ,Organophosphorus Compounds ,medicine ,Organometallic Compounds ,Myocyte ,Animals ,Cells, Cultured ,030304 developmental biology ,0303 health sciences ,Molecular Structure ,010405 organic chemistry ,Chemistry ,Communication ,Depolarization ,General Chemistry ,Controlled release ,0104 chemical sciences ,Mitochondria ,medicine.anatomical_structure ,Biophysics ,Mitochondria targeted - Abstract
Depolarization of an individual mitochondrion or small clusters of mitochondria within cells has been achieved using a photoactivatable probe. The probe is targeted to the matrix of the mitochondrion by an alkyltriphenylphosphonium lipophilic cation and releases the protonophore 2,4-dinitrophenol locally in predetermined regions in response to directed irradiation with UV light via a local photolysis system. This also provides a proof of principle for the general temporally and spatially controlled release of bioactive molecules, pharmacophores, or toxins to mitochondria with tissue, cell, or mitochondrion specificity.
- Published
- 2012