1. X-ray fluorescence detection for serial macromolecular crystallography using a JUNGFRAU pixel detector
- Author
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Bill Pedrini, Bernd Schmitt, Isabelle Martiel, Nadia Opara, Celestino Padeste, Vitaliy A. Guzenko, Meitian Wang, Ezequiel Panepucci, Istvan Mohacsi, S. Redford, Aldo Mozzanica, Dmitry Ozerov, and Filip Leonarski
- Subjects
Nuclear and High Energy Physics ,Materials science ,Astrophysics::High Energy Astrophysical Phenomena ,030303 biophysics ,X-ray fluorescence ,law.invention ,03 medical and health sciences ,Optics ,macromolecular crystallography ,law ,serial crystallography ,Absorption (electromagnetic radiation) ,Instrumentation ,030304 developmental biology ,Physics::Biological Physics ,Quantitative Biology::Biomolecules ,0303 health sciences ,Radiation ,Pixel ,business.industry ,Detector ,equipment and supplies ,Laser ,Research Papers ,Fluorescence ,Synchrotron ,XFELs ,Beamline ,JUNGFRAU detector ,fluorescence ,business ,silicon drift detectors - Abstract
Use of the charge-integrating JUNGFRAU detector for fluorescence detection is investigated, and its applications in macromolecular crystallography at synchrotron and X-ray free-electron laser sources are demonstrated., Detection of heavy elements, such as metals, in macromolecular crystallography (MX) samples by X-ray fluorescence is a function traditionally covered at synchrotron MX beamlines by silicon drift detectors, which cannot be used at X-ray free-electron lasers because of the very short duration of the X-ray pulses. Here it is shown that the hybrid pixel charge-integrating detector JUNGFRAU can fulfill this function when operating in a low-flux regime. The feasibility of precise position determination of micrometre-sized metal marks is also demonstrated, to be used as fiducials for offline prelocation in serial crystallography experiments, based on the specific fluorescence signal measured with JUNGFRAU, both at the synchrotron and at SwissFEL. Finally, the measurement of elemental absorption edges at a synchrotron beamline using JUNGFRAU is also demonstrated.
- Published
- 2020