1. Comparing 'intra operative' tissue engineering strategies for the repair of craniofacial bone defects
- Author
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Florent Espitalier, Soraya Wuillem, Pierre Corre, Vincent Hivernaud, Pierre Weiss, Jérôme Guicheux, Sophie Sourice, S. Portron, Régis Roche, Fanny Grimaud, Richard Pace, P. Pilet, Hélios Bertin, Regenerative Medicine and Skeleton (RMeS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), STEMCIS [Besançon] (Pôle des Microtechniques), Université de Nantes - UFR Odontologie, Université de Nantes (UN), Clinique de Stomatologie et de Chirurgie maxillo-faciale [CHU Nantes], Centre hospitalier universitaire de Nantes (CHU Nantes), Ostéo-articulaire - Tête et cou - Odontologie - Neurochirurgie - Neurotraumatologie [CHU Nantes] (Pôle hospitalo-universitaire PHU4 - OTONN), Laboratoire d'Hematologie [CHU Nantes], Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes), This work was supported the association ‘‘Les Gueules Cassées’’. An author, Vincent Hivernaud received a scholarship grant by the French National Association of Research and Technology and by the STEMCIS company., Jehan, Frederic, Regenerative Medicine and Skeleton research lab (RMeS), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), and Université de Nantes (UN)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)
- Subjects
Pathology ,medicine.medical_specialty ,Bone Regeneration ,Stromal vascular fraction ,Adipose tissue ,Calvaria ,Bone healing ,Bone tissue ,Biphasic calcium phosphate ,03 medical and health sciences ,0302 clinical medicine ,Tissue engineering ,Osteogenesis ,medicine ,Animals ,Humans ,Bone marrow ,030223 otorhinolaryngology ,Bone regeneration ,[SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system ,[SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology ,Tissue Engineering ,business.industry ,[SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology ,030206 dentistry ,Rats ,medicine.anatomical_structure ,Otorhinolaryngology ,[SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system ,Adipose Tissue ,Bone Substitutes ,Surgery ,Oral Surgery ,business ,Nude rat - Abstract
Background In craniofacial reconstruction, the gold standard procedure for bone regeneration is the autologous bone graft (BG). However, this procedure requiring bone harvesting is a source of morbidity. Bone substitutes, such as biphasic calcium phosphate (BCP), represent an interesting alternative but are not sufficient for bone healing in hypoplastic conditions. In such conditions, osteoprogenitors are essential to provide osteoinduction. Previous studies have shown that BCP associated with total bone marrow (TBM) provides same bone reconstruction as bone graft in a rat model of calvaria defect. Furthermore, adipose tissue stromal vascular fraction (SVF) seems to be another promising source of osteoprogenitor cells that can be used intra-operatively. This study aimed to combine, intra-operative BCP-based bone tissue engineering strategies with TBM or SVF from human sources. Methods 5 mm critical-size calvaria defects were performed in 18 nude rat. The defects were filled with intra-operative bone tissue engineering procedures: human BG, human TBM + BCP, human SVF + BCP and, rat TBM + BCP. Animals were sacrificed 8 weeks after implantation and calvaria were processed for histological and radiological examinations. Implanted cells were labelled with a fluorochrome. Results Micro-CT analysis revealed partial repair of bone defect. Only hBG significantly succeeded in healing the defect (43.1%). However, low rate of newly formed bone tissue was observed in all tissue engineering conditions (hTBM, hSVF, ratTBM). Discussion The lack of bone formation observed in this study could possibly be attributed to the model. Conclusion This study combined with a literature analysis show the stringency of the nude rat calvaria model in term of bone regeneration.
- Published
- 2018