1. Preparation of specific antisera to estrone-3-glucuronide and estriol-3-glucuronide.
- Author
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Nambara T, Ohkubo T, Yamaki T, and Shimada K
- Subjects
- Animals, Antigen-Antibody Complex, Cross Reactions, Estriol analysis, Estriol chemical synthesis, Estrone analysis, Estrone chemical synthesis, Haptens, Immune Sera, Indicators and Reagents, Serum Albumin, Bovine, Tritium, Estriol analogs & derivatives, Estrogens, Conjugated (USP) analysis, Estrone analogs & derivatives
- Abstract
The preparation and antigenic properties of estrone-3-glucuronide- and estriol-3-glucuronide-bovine serum albumin conjugates in which the hapten is linked to the carrier protein through an (O-carboxymethyl)oxime bridge at the C-6 position on the steroid nucleus, have been described. Antibodies raised against the two immunogens in the rabbit possessed high specificity to estrone-3-glucuronide and estriol-3-glucuronide, respectively, exhibiting little cross-reactivities with other estrogen conjugates and no cross-reactions with related steroids except for free estrogens, their 3-methyl ethers and 3-sulfates. The cross-reactive antibodies were eliminated by partial immunoadsorption on affinity chromatographic media using the estrone-3-methyl ether 17-(O-carboxymethyl)oxime- and estriol-3-methyl ether 16 (or 17)-hemisuccinate-aminohexyl Sepharose conjugates, respectively. The purified antisera exhibited no cross-reactivities with free estrogens and ring A conjugates of estrone and estriol.
- Published
- 1984
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