1. Buffer Standards for the Physiological pH of the Zwitterionic Compound, ACES from 5 to 55°C.
- Author
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Roy RN, Roy LN, Fuge MS, Roy CN, Himes CA, Bryant PA, Robinson KT, Kaufmann DA 2nd, Grove CH, Ghosh T, and Bwashi A
- Abstract
The values of the second dissociation constant p K
2 and related thermodynamic quantities of [N-(2-acetamido)-2-aminoethanesulfonic acid] (ACES) have already been reported over the temperature range 5 to 55°C including 37°C. This paper reports the paH values of four chloride ion free buffer solutions and eight buffer solutions with I = 0.16 mol·kg-1 , matching closely to that of the physiological sample. Conventional paH values for all twelve buffer solutions from 5 to 55°C, are reported. The residual liquid junction potential correction for two widely used temperatures, 25 and 37°C, has been made. The flowing-junction calomel cell method has been utilized to measure Ej , the liquid junction potential. The operational pH values for four buffer solutions at 25 and 37°C are calculated using the physiological phosphate buffer standard based on NBS/NIST convention. These solutions are recommended as pH standards in the pH range of 6.8 to 7.2 for physiological fluids.- Published
- 2009
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