1. Microfluidic chip electrophoresis for biochemical analysis
- Author
-
Peng Chen, Xizhi Huang, Xiaowen Ou, Bi-Feng Liu, and Shunji Li
- Subjects
Ions ,Gel electrophoresis ,Chromatography ,Chemistry ,010401 analytical chemistry ,Microfluidics ,Electrophoresis, Capillary ,Proteins ,Filtration and Separation ,Electrochemical Techniques ,02 engineering and technology ,Microfluidic Analytical Techniques ,Dielectrophoresis ,021001 nanoscience & nanotechnology ,Mass spectrometry ,01 natural sciences ,0104 chemical sciences ,Analytical Chemistry ,Electrophoresis ,Capillary electrophoresis ,Microfluidic chip ,Nucleic Acids ,Amino Acids ,0210 nano-technology - Abstract
Microfluidic chip electrophoresis has been widely employed for separation of various biochemical species owing to its advantages of low sample consumption, low cost, fast analysis, high throughput, and integration capability. In this article, we reviewed the development of four different modes of microfluidics-based electrophoresis technologies including capillary electrophoresis, gel electrophoresis, dielectrophoresis, and field (electric) flow fractionation. Coupling detection schemes on microfluidic electrophoresis platform were also reviewed such as optical, electrochemical, and mass spectrometry method. We further discussed the innovative applications of microfluidic electrophoresis for biomacromolecules (nucleic acids and proteins), biochemical small molecules (amino acids, metabolites, ions, etc.), and bioparticles (cells and pathogens) analysis. The future direction of microfluidic chip electrophoresis was predicted.
- Published
- 2019