1. Monitoring of cefepime in human serum and plasma by micellar electrokinetic capillary chromatography: Improvement of sample preparation and validation by liquid chromatography coupled to mass spectrometry
- Author
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Regula Theurillat, Parham Sendi, Wolfgang Thormann, and Nela Šestáková
- Subjects
0301 basic medicine ,Cefepime ,Electrospray ionization ,030106 microbiology ,610 Medicine & health ,Filtration and Separation ,Mass spectrometry ,01 natural sciences ,Mass Spectrometry ,Micellar electrokinetic chromatography ,Analytical Chemistry ,03 medical and health sciences ,Capillary electrophoresis ,Liquid chromatography–mass spectrometry ,medicine ,Humans ,Protein precipitation ,Sample preparation ,Chromatography, Micellar Electrokinetic Capillary ,Chromatography ,Chemistry ,010401 analytical chemistry ,Cephalosporins ,0104 chemical sciences ,570 Life sciences ,biology ,Chromatography, Liquid ,medicine.drug - Abstract
Cefepime monitoring in deproteinized human serum and plasma by micellar electrokinetic capillary chromatography and liquid chromatography coupled to mass spectrometry in presence of other drugs is reported. For micellar electrokinetic capillary chromatography, sample preparation comprised dodecylsulfate protein precipitation at pH 4.5 using an increased buffer concentration compared to that of a previous assay and removal of hydrophobic compounds with dichloromethane. This provided robust conditions for cefepime analysis in the presence of sulfamethoxazole and thus enabled its determination in samples of patients that receive co-trimoxazole. The liquid chromatography assay is based upon use of a column with a pentafluorophenyl-propyl modified and multi-endcapped stationary phase and the coupling to electrospray ionization with a single quadrupole detector. The performances of both assays with multi-level internal calibration were assessed with calibration and control samples and both assays were determined to be robust. Cefepime levels monitored by micellar electrokinetic capillary chromatography in samples from patients that were treated with cefepime only and with cefepime and co-trimoxazole were found to compare well with those obtained by liquid chromatography coupled to mass spectrometry. Cefepime drug levels determined by micellar electrokinetic capillary chromatography could thereby be validated. This article is protected by copyright. All rights reserved
- Published
- 2017