1. Rapid preparative isolation of a new phenylpropanoid glycoside and four minor compounds from Sparganium stoloniferum using high-speed counter-current chromatography as a fractionation tool
- Author
-
Shuguang Guan, De-An Guo, Shu-Hong Guan, Wanying Wu, Ruihong Feng, Lianxue Zhang, Xuan Liu, Jiling Guo, Wenbo Yu, Hao Wu, Baohong Jiang, Wei Man, and Min Yang
- Subjects
chemistry.chemical_classification ,Chromatography ,Phenylpropanoid ,Plant Extracts ,Chemistry ,Glycoside ,Filtration and Separation ,Fractionation ,High-performance liquid chromatography ,Analytical Chemistry ,Rhizome ,Magnoliopsida ,chemistry.chemical_compound ,Countercurrent chromatography ,Yield (chemistry) ,Vanillic acid ,Organic chemistry ,Glycosides ,Countercurrent Distribution ,Chromatography, High Pressure Liquid - Abstract
A rapid high-speed counter-current chromatography (HSCCC) method was used to isolate five minor compounds from rhizome of Sparganium stoloniferum namely San Leng in Chinese, including two phenylpropanoid glycosides, sparganiaside A (1) and 1-O-feruloyl-3-p-coumaroylglycerol (2), and three aromatic acids, vanillic acid (3), p-hydroxylcinnamic acid (4), and p-hydroxybenzoic acid (5), of which, compound 1 was a new one. Five compounds were preparatively enriched at top efficiency by one-step HSCCC operation in the isolation procedure. A suitable solvent system composed of chloroform-methanol-water (4:3.5:1.8, v/v/v) was used. And the operation time was less than 4 h. The purities of compounds (1-5) in the enriched fractions were determined to be 75.8%, 66.3%, 90.6%, 79.9%, and 98.2%, respectively. The mean recoveries of the five compounds were 84.8%, 87.3%, 81.8%, 90.3%, and 92.7%, respectively. Compounds 1-4 were further purified by semi-preparative high-performance liquid chromatography (HPLC). This is the first report on the use of HSCCC as a fractionation tool for preparative isolation of minor compounds from S. stoloniferum. The method was proved to be rapid, convenient, high yield, and low cost. HSCCC was shown to be a quick and effective tool in isolation of natural products even though the compounds were not abundant.
- Published
- 2012