Search

Your search keyword '"Szumala-Kakol A"' showing total 11 results
Start Over Author "Szumala-Kakol A" Journal journal of reproductive immunology
11 results on '"Szumala-Kakol A"'

3. In vitro reconstruction of inflammatory reaction in human semen: effect on sperm DNA fragmentation

Author

Maciej Kurpisz, Grzegorz Dworacki, Dorota Sanocka, Monika Fraczek, and Anna Szumala-Kakol

Subjects
Male, DNA damage, Bacteroidaceae, Immunology, Semen, DNA Fragmentation, Semen inflammation, Biology, Obstetrics and Gynaecology, Bacteroidaceae Infections, Escherichia coli, Humans, Immunology and Allergy, Comet assay, Cells, Cultured, Escherichia coli Infections, Infertility, Male, Differential centrifugation, TUNEL assay, Obstetrics and Gynecology, Molecular biology, Sperm, Reproductive Medicine, Sperm Motility, DNA fragmentation, Cytokines, Inflammation Mediators, Percoll
Abstract

The study was aimed at evaluating an in vitro induction of DNA damage in three sperm subpopulations exposed to selected inflammatory mediators, such as leukocytes, two combinations of pro-inflammatory cytokines (interleukin [IL]-6+IL-8 and IL-12+IL-18) and two bacterial strains (Escherichia coli and Bacteroides ureolyticus). Semen samples from normozoospermic volunteers were differentiated by swim-up (swim-up fraction) and Percoll gradient procedures (90% and 47% Percoll fractions). Leukocytes were isolated from the whole heparinized blood using the density gradient centrifugation technique. DNA fragmentation in sperm fractions was evaluated using flow cytometry with TUNEL labeling and Comet assay. Out of the inflammatory factors tested, bacteria were found to have a greatest toxic effect on sperm DNA, especially in fractions isolated by Percoll gradient, compared with untreated cells (P

Published
2013
Full Text
View/download PDF

5. Relationship between semen infection and sperm quality—in vitro study

Author

Monika Fraczek, Maria Laszczyńska, Małgorzata Piasecka, Anna Szumala-Kakol, Dariusz Gaczarzewicz, Maciej Kurpisz, and Anna Kazienko

Subjects
Andrology, Reproductive Medicine, Immunology, Obstetrics and Gynecology, Immunology and Allergy, In vitro study, Semen, Biology, Sperm quality
Published
2011

11. Innate immunity is linked with oxidative stress and may lead to male infertility

Author

Monika Fraczek, Piotr Jedrzejczak, Maciej Kurpisz, and Anna Szumala-Kakol

Subjects
Innate immune system, Reproductive Medicine, business.industry, Immunology, Obstetrics and Gynecology, Immunology and Allergy, Medicine, business, medicine.disease_cause, medicine.disease, Oxidative stress, Male infertility
Published
2007

Catalog

Books, media, physical & digital resources
See catalog results