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Your search keyword '"Laiakis, Evagelia C."' showing total 9 results
Start Over Author "Laiakis, Evagelia C." Journal journal of proteome research
9 results on '"Laiakis, Evagelia C."'

1. Biofluid Metabolomics of Mice Exposed to External Low-Dose Rate Radiation in a Novel Irradiation System, the Variable Dose-Rate External 137Cs Irradiator

Author

Pannkuk, Evan L., primary, Laiakis, Evagelia C., additional, Girgis, Michael, additional, Garty, Guy Y., additional, Morton, Shad R., additional, Pujol-Canadell, Monica, additional, Ghandhi, Shanaz A., additional, Amundson, Sally A., additional, Brenner, David J., additional, and Fornace, Albert J., additional

Published
2021
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3. Fabric Phase Sorptive Extraction—A Metabolomic Preprocessing Approach for Ionizing Radiation Exposure Assessment

Author

Taraboletti, Alexandra, primary, Goudarzi, Maryam, additional, Kabir, Abuzar, additional, Moon, Bo-Hyun, additional, Laiakis, Evagelia C., additional, Lacombe, Jerome, additional, Ake, Pelagie, additional, Shoishiro, Sueoka, additional, Brenner, David, additional, Fornace, Albert J., additional, and Zenhausern, Frederic, additional

Published
2019
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4. Liquid Chromatography–Mass Spectrometry-Based Metabolomics of Nonhuman Primates after 4 Gy Total Body Radiation Exposure: Global Effects and Targeted Panels

Author

Pannkuk, Evan L., primary, Laiakis, Evagelia C., additional, Gill, Kirandeep, additional, Jain, Shreyans K., additional, Mehta, Khyati Y., additional, Nishita, Denise, additional, Bujold, Kim, additional, Bakke, James, additional, Gahagen, Janet, additional, Authier, Simon, additional, Chang, Polly, additional, and Fornace, Albert J., additional

Published
2019
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5. A Serum Small Molecule Biosignature of Radiation Exposure from Total Body Irradiated Patients

Author

Laiakis, Evagelia C., Pannkuk, Evan L., Chauthe, Siddheshwar Kisan, Wang, Yi-Wen, Lian, Ming, Mak, Tytus D., Barker, Christopher A., Astarita, Giuseppe, and Fornace, Albert J.

Abstract

The potential for radiological accidents and nuclear terrorism has increased the need for the development of new rapid biodosimetry methods. In addition, in a clinical setting the issue of an individual’s radiosensitivity should be taken into consideration during radiotherapy. We utilized metabolomics and lipidomics to investigate changes of metabolites in serum samples following exposure to total body ionizing radiation in humans. Serum was collected prior to irradiation, at 3–8 h after a single dose of 1.25–2 Gy, and at 24 h with a total delivered dose of 2–3.75 Gy. Metabolomics revealed perturbations in glycerophosphocholine, phenylalanine, ubiquinone Q2, and oxalic acid. Alterations were observed in circulating levels of lipids from monoacylglycerol, triacylglycerol, phosphatidylcholine, and phosphatidylglycerol lipid classes. Polyunsaturated fatty acids were some of the most dysregulated lipids, with increased levels linked to proinflammatory processes. A targeted metabolomics approach for eicosanoids was also employed. The results showed a rapid response for proinflammatory eicosanoids, with a dampening of the signal at the later time point. Sex differences were observed in the markers from the untargeted approach but not the targeted method. The ability to identify and quantify small molecules in blood can therefore be utilized to monitor radiation exposure in human populations.

Published
2024
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9. Biofluid Metabolomics of Mice Exposed to External Low-Dose Rate Radiation in a Novel Irradiation System, the Variable Dose-Rate External 137 Cs Irradiator.

Author

Pannkuk EL, Laiakis EC, Girgis M, Garty GY, Morton SR, Pujol-Canadell M, Ghandhi SA, Amundson SA, Brenner DJ, and Fornace AJ Jr

Subjects
Animals, Biomarkers, Dose-Response Relationship, Radiation, Female, Male, Mass Spectrometry, Mice, Cesium Radioisotopes, Metabolomics methods
Abstract

An important component of ionizing radiation (IR) exposure after a radiological incident may include low-dose rate (LDR) exposures either externally or internally, such as from 137 Cs deposition. In this study, a novel irradiation system, VAriable Dose-rate External 137 Cs irradiatoR (VADER), was used to expose male and female mice to a variable LDR irradiation over a 30 d time span to simulate fall-out-type exposures in addition to biofluid collection from a reference dose rate (0.8 Gy/min). Radiation markers were identified by untargeted metabolomics and random forests. Mice exposed to LDR exposures were successfully identified from control groups based on their urine and serum metabolite profiles. In addition to metabolites commonly perturbed after IR exposure, we identified and validated a novel metabolite (hexosamine-valine-isoleucine-OH) that increased up to 150-fold after LDR and 80-fold after conventional exposures in urine. A multiplex panel consisting of hexosamine-valine-isoleucine-OH with other urinary metabolites ( N 6, N 6, N 6-trimethyllysine, carnitine, 1-methylnicotinamide, and α-ketoglutaric acid) achieved robust classification performance using receiver operating characteristic curve analysis, irrespective of the dose rate or sex. These results show that in terms of biodosimetry, dysregulated energy metabolism is associated with IR exposure for both LDR and conventional IR exposures. These mass spectrometry data have been deposited to the NIH data repository via Metabolomics Workbench with study IDs ST001790, ST001791, ST001792, ST001793, and ST001806.

Published
2021
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