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Purpose: The purpose of this study was to evaluate the accuracy of polyetheretherketone (PEEK) based post and core restorations using heat-pressed and computer-aided design and computer-aided manufacturing (CAD-CAM) methods., Materials and Methods: Post and core patterns (n = 10) were fabricated using an acrylic pattern resin and each pattern was scanned to generate a resin pattern standard tessellation file (STL-R) to be used as the standard control group for the 3D profilometric analysis. The STL-R files were loaded into a computer-aided manufacturing (CAM) machine and a milling disk made of a modified PEEK-based polymer with 20% ceramic fillers was milled into post and core restorations. Each original post and core resin pattern was sprued, invested and pressed using pellets made of the same PEEK material and a PEEK pressing unit. The resulting post and core restorations from the CAD-CAM and heat-pressed methods were scanned to generate STL-M (milled) and STL-P (heat-pressed) files, respectively. STL-M and STL-P files were digitally aligned and compared to STL-R files using a 3D analysis software. The 3D spatial distribution deviation was exported, and the statistical analysis was performed using the 95% CI deviation distribution from the Gaussian function subtraction. The statistical outcome for each group was merged and a 3D color-coded map was created showing the average deviation (α = 0.05)., Results: The heat-pressed post and core restoration showed 79 ±6% of the areas within ±50 µm while the CAD-CAM post-and-core restoration showed 66 ±9%. The CAD-CAM post and core restorations show 6 ±5% of the areas above the ideal for marginal adaptation limit threshold of 200 µm, while only 0.7 ±0.7% was found for the heat-pressed., Conclusions: The heat-pressed method to manufacture PEEK-based post and core restorations showed higher accuracy than the CAD-CAM method., (© 2021 by the American College of Prosthodontists.)