1. Short term treatment of secondary lymphedema with hyaluronidase injections reduces mouse hindlimb lymphedema.
- Author
-
Dalaei F, Bucan A, Wiinholt A, Jørgensen MG, Hansen CR, Baun C, Hvidsten S, Hejbøl EK, Schrøder HD, and Sørensen JA
- Subjects
- Mice, Female, Humans, Animals, X-Ray Microtomography adverse effects, Mice, Inbred C57BL, Hindlimb, Lower Extremity, Lymphoscintigraphy adverse effects, Chronic Disease, Hyaluronoglucosaminidase pharmacology, Hyaluronoglucosaminidase therapeutic use, Lymphedema diagnostic imaging, Lymphedema drug therapy, Lymphedema etiology
- Abstract
Lymphedema is a common complication following breast cancer treatment with axillary lymphadenectomy and radiotherapy. Currently, there is no curative treatment for this disease, hence there is a need for new therapeutic suggestions. The aim of this study was to investigate the effect of hyaluronidase (HYAL) injections after inducing hindlimb lymphedema in 36 female C57BL/6 mice. HYAL injections were administered every second day for 14 days in three groups: (1) HYAL for 1 week followed by saline for 1 week, (2) HYAL for 2 weeks, and (3) saline injections for 2 weeks. Volume of the lymphedema limb was weekly assessed with micro-computed tomography (μ-CT) scans for a total course of 6 weeks. Lymph vessel morphometry was assessed in the end of the study after staining cross-sections of the hindlimb for anti-LYVE-1 blindly. Lymphatic function was assessed by lymphoscintigraphy to assess lymphatic clearance. There was a significant reduction of the volume of lymphedema in mice treated with HYAL-7 compared with mice treated with HYAL-14 (p < 0.05) and saline (p < 0.05). No differences were detected in lymph vessel morphometry and the lymphoscintigraphy between groups. Short-term treatment with HYAL-7 might be a potential therapeutic suggestion for secondary lymphedema induced in mouse hindlimbs. In the future, clinical studies are needed to investigate the potential of HYAL treatment in human beings.
- Published
- 2023
- Full Text
- View/download PDF