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The aim of this study is to improve the propagation of Impatiens balsamina, L by an efficient protocol for in vitro propagation was successfully established through tissue culture technique. Cotyledonary node with sections of cotyledon, section of cotyledons and hypocotyls were derived from 21 days in vitro germinating seeds after surface sterilization. They were cultured for shoot multiplication and callus induction on MS medium fortified with various concentrations of BAP, TDZ or combination between BAP and NAA. It was determined that cotyledonary node with sections of cotyledon on MS medium supplemented with 4.5 mg/L BAP for maximum shoots number (4.33shoots) and callus induction 3.78. The best shoot length (3.08 cm) within 8 weeks from regeneration was achieved on MS medium fortified with 1.0 mg/L BAP + 0.5 mg/L NAA. The number of shoots formed per explants increased by re-culturing for six weeks on the same medium (4.5 mg/L BAP alone), since it produced (9 shoots). While the best shoot length (4cm) was obtained by sub culturing the explants on MS medium received 1.0 mg/L BAP + 0.5 mg/L NAA to MS without growth regulators after six weeks. The best callus fresh weight (9.32 g) and callus volume (7.76 cm3) were recorded with using TDZ at 3.0 mg/L that sub cultured on medium fortified with 4.5 mg/L BAP. In addition the highest dry weight (0.81 g) was obtained by re - culturing on the same medium that contained with 4.5 mg/L BAP.

This study was conducted at the Experimental Station and Laboratory of Vegetable and Floriculture Department, Fac. of Agric., Mansoura Univ., Egypt, during 2015/2016 and 2016/2017 seasons to investigate the effect of some natural products and some alternative chemical growth regulators on rooting response‚ growth and chemical composition of cuttings of rosemary (Rosmarinus officinalis L.) plants. The layout of the experiment was completely randomized block design (CRBD) with three replications. The obtained results could be summarized as the following: It is worthy to mention that the highest values of all studied vegetative growth characters and chemical constituents of rosemary were resulted from treating terminal cuttings with coconut milk at the highest rate (75%) in both seasons. With exception, fresh weight of shoots, which, obtained when treating terminal cuttings with seaweed extract at the rate of 50 % and total phenols percentage, which obtained from control treatment (treating cuttings with water only) in both seasons. Using natural products such as coconut milk, seaweed extraction, yeast extract and honey bee were better than using chemical growth regulators like Indole butyric acid (IBA) in both seasons. It can be concluded that treating terminal cuttings of rosemary (Rosmarinus officinalis L.) with coconut milk at the rate of 75 % as natural growth regulator in order to enhance rooting‚ vegetative growth and chemical constituents.