1. Asparagine and sugars are both required to sustain secondary axis elongation after bud outgrowth in Rosa hybrida
- Author
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Alain Vian, Pierre-Maxime Furet, Anis M. Limami, Lukáš Spíchal, Vincent Billard, Vincent Guérin, Marie-Anne Le Moigne, Hanaé Roman, Jérémy Lothier, Sylvie Citerne, Annette Morvan-Bertrand, Anita Lebrec, Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Centre de Recherches Historiques de l'Ouest (CERHIO), Le Mans Université (UM)-Université d'Angers (UA)-Université de Bretagne Sud (UBS)-Université de Rennes 2 (UR2), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Ecophysiologie Végétale, Agronomie et Nutritions (EVA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Ministere de l'Enseignement Superieur et de la Recherche, France, National Program of Sustainability from the Ministry of Education, Youth and Sports of the Czech Republic : LO1204, and AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA)
- Subjects
0106 biological sciences ,0301 basic medicine ,Sucrose ,Cytokinins ,Physiology ,Nitrogen ,Plant Science ,Rosa ,01 natural sciences ,Plant Roots ,03 medical and health sciences ,chemistry.chemical_compound ,Plant Growth Regulators ,Axillary bud ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,Asparagine ,Axis elongation ,ComputingMilieux_MISCELLANEOUS ,chemistry.chemical_classification ,Xylem ,Biological Transport ,Amino acid ,030104 developmental biology ,chemistry ,Biochemistry ,Cytokinin ,Amino acids ,Elongation ,Bud outgrowth ,Agronomy and Crop Science ,010606 plant biology & botany - Abstract
International audience; Nitrogen is required for optimal plant growth, especially in young organs such as secondary axes (axes II) after axillary bud outgrowth. Several studies have shown an increase of nitrogen concentration in xylem sap concomitantly with bud outgrowth, but the relation between nitrogen, sugars and plant hormones in axis II still remains unclear. We investigated in Rosa hybrida the involvement of nitrogen nutrition in axis II elongation in relation with sugars and cytokinins using N-15-labeled nitrate and sugars, amino acids and cytokinin quantifications. Besides, we measured the effect of the exogenous supply of these compounds on axis II elongation using in vitro excised bud culture. We demonstrated that nitrogen in the axis II comes mainly from new root uptake after decapitation. Asparagine, which concentration increases in sap exudates and tissues during axis II elongation, was the sole amino acid able to sustain an efficient elongation in vitro when supplied in combination with sucrose.
- Published
- 2017