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The presence and distribution of Tomato spotted wilt virus (TSWV) on different vegetable, ornamental, tobacco plants and weeds was investigated in the open field and greenhouses in different locations of Montenegro. DAS ELISA was used to identify the virus in 2072 samples collected from 2007 to 2009. TSWV was detected in two samples collected in 2007, none of the samples collected in 2008 and 50 samples collected in 2009. The sensitivity and specificity of four different previously described primer sets for TSWV detection were assessed by RT-PCR. The N gene sequences were compared with those of 20 new isolates from Montenegro, Serbia, Italy and Brazil and 27 others retrieved from the NCBI database. Nucleotide sequences comparison revealed high level of similarity (97-99,9%) between Montenegrin and TSWV isolates from other European countries. Phylogenetic analysis of the N gene revealed seven distinct subgroups, showing that the investigated isolates clustered with those from the same geographic region, with the exception of one Montenegrin (Is-103) and one Italian isolate (Gb-21). [ABSTRACT FROM AUTHOR]

Tomato spotted wilt virus (TSWV) was isolated from naturally infected lettuce grown in Jordan. This virus isolate (TSWV-J) was identified based on transmission studies, host range reaction, and serological tests. RT-PCR was used for the detection of TSWV in infected plant tissues. Total RNA isolated from infected lettuce plants was subjected to RT-PCR using primers specific to the nucleocapsid (N) gene of TSWV and the amplified 620 bp product was cloned and sequenced. Nucleotide sequence and phylogenetic analysis of the RTPCR amplicon confirmed the Jordanian virus as an isolate of TSWV. Furthermore, the genetic diversity of TSWV in Jordan was investigated. The TSWV isolates shared high nucleotide sequence identity (93-99%) with those from other countries. To our knowledge, this is the first report of TSWV infection on lettuce in Jordan. [ABSTRACT FROM AUTHOR]