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Ti-24Nb-4Zr-8Sn (TNZS) as an important material in biomedical field, has ushered in broader application space and proposed higher performance requirements. This paper presents the experimental investigation of the effecct of TiO2 content on the microstructure and properties of TNZS prepared by powder metallurgy, aiming to optimize the additional amount of TiO2 (0wt.%, 3wt.%, 6wt.% and 9wt.%), improve the microstructure uniformity and properties. The phase, microstructure, hardness, elastic modulus were measured. The results indicate that nTiO2/TNZS alloy was composed of α-Ti, β-Ti, Rutile TiO2 and Anatase TiO2. Meanwhile, the diffraction peals intensity of α-Ti and Rutile TiO2 were enhanced obviously with the increasing of TiO2 content. With the increase of TiO2, the size of grain was refined and the distribution became homogenized. As the TiO2 content rises, the hardness first decrease and then increase. The results show that the micro-hardness of nTiO2/TNZS alloys were 579.8 HV, 452.9 HV, 983.2 HV and 1219.9 HV, the micro-hardness of 9wt.%TiO2/TNZS alloy reach the maximum. The elastic modulus of nTiO2/TNZS alloys were 43.29 GPa, 50.44 GPa, 57.62 GPa and 85.37 GPa.