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The association between contextual interference effect (CIE) and kinematic measures has been little investigated. The purpose of this study was to investigate the CIE on velocity and acceleration kinematic profiles of upper limb. Thirty-two subjects (23,7 ± 3,3 years) were assigned to groups of blocked practice (GPB) and random practice (GPA). The task consisted in achieve three targets in three specific sequences as quickly and accurately as possible. The study was designed in acquisition phase and transfer test. The variables of performance were reaction time, movement time and response time and the kinematic variables were peak of speed, relative time to peak of speed and number of peak acceleration points. The main findings showed that GPA showed lower movement time and response time than GPB. Regression analysis indicated that change in peak velocity during practice was associated to the change of the reaction time from practice to the transfer test. As peak velocity is a measure related to preprogramming, it is suggested that central control was essential to the CIE. [ABSTRACT FROM AUTHOR]