1. Hydroalcoholic extract of Araucaria sp. brown propolis alleviates ulcerative colitis induced by TNBS in rats by reducing inflammatory cell infiltration and oxidative damage.
- Author
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Cury BJ, Jerônimo DT, da Silva LM, Farias de Queiroz E Silva T, França TCS, Dos Santos AC, Andriolo IRL, Santin JR, Benvenutti L, Vaz CR, Santos MFC, Kenupp JB, and da Silva LM
- Subjects
- Animals, Male, Rats, Colon drug effects, Colon pathology, Colon metabolism, Peroxidase metabolism, Glutathione metabolism, Superoxide Dismutase metabolism, Anti-Inflammatory Agents pharmacology, Anti-Inflammatory Agents isolation & purification, Disease Models, Animal, Dexamethasone pharmacology, Tracheophyta chemistry, Catalase metabolism, Dose-Response Relationship, Drug, Antioxidants pharmacology, Glutathione Transferase metabolism, Colitis, Ulcerative drug therapy, Colitis, Ulcerative chemically induced, Colitis, Ulcerative pathology, Colitis, Ulcerative metabolism, Trinitrobenzenesulfonic Acid, Propolis pharmacology, Oxidative Stress drug effects, Plant Extracts pharmacology, Rats, Wistar, Malondialdehyde metabolism
- Abstract
Objective: To investigate the effects of Araucaria sp. brown propolis (ABP) against trinitrobenzenesulfonic acid (TNBS)-induced colitis in rats., Methods: Animals received vehicle (1% DMSO, 1 ml/kg) or hydroalcoholic extract of ABP (hydroalcoholic extract of Araucaria sp. brown propolis (HEABP), 30, 100, and 300 mg/kg) orally, or dexamethasone (25 mg/kg, s.c.) for 5 days. On day 4, the animals received intracolonic TNBS (150 mg/kg), on day 6 they were euthanized. The weight of the animals, the macroscopic and microscopic colonic damage, reduced glutathione (GSH) and malondialdehyde (MDA) levels, and the activity of glutathione S-transferase (GST), catalase (CAT), superoxide dismutase (SOD), and myeloperoxidase (MPO) were measured in colon homogenate. The action of HEABP and two isolated compounds in neutrophil migration was recorded., Key Findings: HEABP (100 and 300 mg/kg), but not dexamethasone, decreased colonic lesion, and increased colonic mucin staining. In parallel, HEABP decreased MDA and restored GSH levels and the activity of SOD, CAT, and GST in the colon. A dose-dependent inhibition of MPO activity was observed (LogIC50 = 1.9). Moreover, HEBPA and the junicedric and abietic acids inhibited the neutrophil chemotaxis in vitro and HEBPA reduced neutrophil migration in vivo., Conclusion: HEABP may be promising in the therapies for inflammatory bowel diseases, reducing oxidative and inflammatory damage, especially mediated by neutrophils., (© The Author(s) 2024. Published by Oxford University Press on behalf of the Royal Pharmaceutical Society. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)
- Published
- 2024
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