1. Optimization of LC method for the quantification of doxorubicin in plasma and urine samples in view of pharmacokinetic, biomedical and drug monitoring therapy studies
- Author
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Natalia Miękus, Elżbieta Adamkiewicz-Drożyńska, Tomasz Bączek, Ilona Olędzka, Piotr Kowalski, Ewa Bien, Alina Plenis, Malgorzata Krawczyk, and Olga Maliszewska
- Subjects
Male ,Analyte ,Adolescent ,Anthracycline ,Clinical Biochemistry ,Pharmaceutical Science ,Urine ,01 natural sciences ,Fluorescence ,Analytical Chemistry ,03 medical and health sciences ,0302 clinical medicine ,Pharmacokinetics ,Limit of Detection ,Neoplasms ,Occupational Exposure ,Antineoplastic Combined Chemotherapy Protocols ,Drug Discovery ,medicine ,Humans ,Doxorubicin ,Chromatography, High Pressure Liquid ,Spectroscopy ,Etoposide ,Detection limit ,Daunorubicin Hydrochloride ,Antibiotics, Antineoplastic ,Chromatography ,Chemistry ,Daunorubicin ,Solid Phase Extraction ,010401 analytical chemistry ,Reproducibility of Results ,Pediatric cancer ,0104 chemical sciences ,Personnel, Hospital ,Vincristine ,030220 oncology & carcinogenesis ,Prednisone ,Drug Monitoring ,medicine.drug - Abstract
A simple, rapid, reliable and sensitive method based on liquid chromatography with fluorescence detection (LC-FL) for the quantification of doxorubicin (DOX) in human plasma and urine samples was developed. The assay was carried out after the solid-phase extraction procedure (SPE) with hydrophilic-lipophilic balance (HLB) cartridges, and with daunorubicin hydrochloride (DAU) used as the internal standard. Chromatographic separation was performed on a Discovery HS C18 column in isocratic elution mode, and the detection of the analytes set at excitation and emission wavelengths of 487 and 555 nm, respectively. The developed LC-FL method has been validated for accuracy, precision, selectivity, linearity, recovery and stability. The limits of detection and quantification for DOX were 0.5 and 1 ng/mL in both biological fluids, respectively. Linearity was confirmed in the range of 1–1000 ng/mL and 0.001–25 μg/mL in plasma and urine samples, respectively, with a correlation coefficient greater than 0.9994. The proposed LC-FL method is selective, precise and accurate, and has been successfully applied for drug monitoring in pediatric cancer patients treated with DOX as a component of OEPA (Oncovin (Vincristine)-Etoposide-Prednisone-Adriamycin) and IOA (Ifosfamide-Oncovin-Adriamycin) chemotherapeutic schemes. Moreover, real exposure of hospital personnel to the anthracycline drugs in plasma and urine was evaluated in clinical practice.
- Published
- 2018