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The upper Permian Longtan Formation (LTF) shale is a new shale gas exploration and development focus in China, which was formed in a transitional depositional environment. Because of the difference in the sedimentary environment between marine-continental transitional shale and marine shale, the enrichment characteristics and mechanism of organic matter (OM) are very different. Because of a weak research foundation, the enrichment mechanism of OM in the LTF shale in the Sichuan Basin is still unclear. In this paper, shale samples of the upper Permian LTF from the Well F5 were collected, and the abundance, source and sedimentary environment of organic matter were studied by using paleoclimate, detrital input, redox, paleoproductivity and sedimentation rate parameters. The results show that the TOC content of the LTF shale is high (average = 3.04%) and reaches the high to overmature stage (average = 2.46%), indicating a favorable source rock. The LTF shale was deposited in a humid and hot climate, which was conducive to the growth and prosperity of a large number of plants. The source of organic matter was mainly terrestrial plant fragments. The sedimentary environment of the LTF shale was oxic, it had a high sedimentation rate, which reduced the oxidation rate of OM, promoted the rapid deposition of OM, and ensured the preservation of organic matter. In summary, the enrichment of OM of LTF shale in the Sichuan Basin was affected by the sedimentary environment, which was complicated. The main controlling factors were detrital input, redox conditions and sedimentation rate. • The geochemical characteristics of Permian Longtan Formation (PLTF) shale in eastern Sichuan are analyzed. • The sedimentary paleoenvironment of PLTF shale in eastern Sichuan is reconstructed. • The main controlling factors of organic matter enrichment of PLTF in eastern Sichuan are studied for the first time. [ABSTRACT FROM AUTHOR]