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An enzymatic method, SK-013, was developed for rapid detection of the peptidase activity in subgingival plaque samples. This method was found to have specificity for Porphyromonas gingivalis, Treponema denticola, Bacteroides forsythus, and some Capnocytophaga strains. The purpose of this study was to determines whether SK-013 could indicate the presence of periodontopathic bacteria including T. denticola, P. gingivalis and B. forsythus, which produce trypsin-lime enzymes. Subgingival plaque samples were taken from 10 clinically healthy sites and 30 periodontally diseased sites with 3 paper points. SK-013 activity of plaque samples was assayed, and the numbers of T. denticola, P. gingivalis and B. forsythus in the sample were counted by immunofluorescence technique. In diseases sites, the SK-013 activity was significantly correlated with clinical parameters such as Gingival Index. Plaque Index, probing depth and bleeding on probing. A significant correlation was found between the presence of these organisms and SK-013 activity. Correlation coefficients between the presence of T. denticola and SK-013 activity were higher than those with other organisms. These findings indicate that the SK-013 is useful as an indicator of cell population of T. denticola, P. gingivalis and B. forsythus in subgingival plaque. [ABSTRACT FROM AUTHOR]

Porphyromonas gingivalis, Bacteroides forsythus, and Treponema denticola have been found to predominate in peridontal pockets of patients with adult periodontitis. These microorganisms hydrolyze the synthetic peptide N-benezoyl-DL-arginine-2-napththylamide (BANA). In this study, we developed an enzymatic method , designated SK-013, to detect the existence of these microorganisms in subgingival plaque bacteria. This enzymatic method was based on the observation of the hydrolysis of N-carbobenzoxy-glycyl-glycyl-arginyl-3,5-dibromo-4-hydroxyaniline (N-CBz-GLy-ARg-DBHA) and made more sensitive by adding an enhancing system. The SK-013 was specifically positive for P. gingivalis, B. forsythus. T. denticola, and some strains of Capnocytophaga species, but was not specific for any of the other bacterial strains tested. This SK-013 system may be valuable for detection and quantification of periodontal disease-associated bacteria in sub-gingival plaque and thus for diagnosis of peridontal infections. [ABSTRACT FROM AUTHOR]

The article presents a list of persons who served as peer reviewers for the journal during 2019.

A list of individuals that reviewed articles for the journal between December of 2013 and November of 2014 is presented which includes Hermann Agis, Ivan Darby and James Fine.

The article presents a list of reviewers, of the Journal of Periodontal Research, who the author would like to thank for their assistance in reviewing manuscripts between 1 January 2012 and 30 November 2012.