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Start Over Author "Takayama, Koji" Journal journal of orthopaedic research
11 results on '"Takayama, Koji"'

4. Gelatin hydrogels with eicosapentaenoic acid can prevent osteoarthritis progression in vivo in a mouse model

Author

Tsubosaka, Masanori, primary, Kihara, Shinsuke, additional, Hayashi, Shinya, additional, Nagata, Junpei, additional, Kuwahara, Toshie, additional, Fujita, Masahiro, additional, Kikuchi, Kenichi, additional, Takashima, Yoshinori, additional, Kamenaga, Tomoyuki, additional, Kuroda, Yuichi, additional, Takeuchi, Kazuhiro, additional, Fukuda, Koji, additional, Takayama, Koji, additional, Hashimoto, Shingo, additional, Matsumoto, Tomoyuki, additional, Niikura, Takahiro, additional, Tabata, Yasuhiko, additional, and Kuroda, Ryosuke, additional

Published
2020
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6. mTOR signaling plays a critical role in the defects observed in muscle-derived stem/progenitor cells isolated from a murine model of accelerated aging

Author

Takayama, Koji, Kawakami, Yohei, Lavasani, Mitra, Mu, Xiaodong, Cummins, James H., Yurube, Takashi, Kuroda, Ryosuke, Kurosaka, Masahiro, Fu, Freddie H., Robbins, Paul D., Niedernhofer, Laura J., and Huard, Johnny

Subjects
senescence, biology, muscle, stem cells, aging, mTOR, ERCC1-XPF, progeria
Abstract

Mice expressing reduced levels of ERCC1-XPF (Ercc1(-/Δ) mice) demonstrate premature onset of age-related changes due to decreased repair of DNA damage. Muscle-derived stem/progenitor cells (MDSPCs) isolated from Ercc1(-/Δ) mice have an impaired capacity for cell differentiation. The mammalian target of rapamycin (mTOR) is a critical regulator of cell growth in response to nutrient, hormone, and oxygen levels. Inhibition of the mTOR pathway extends the lifespan of several species. Here, we examined the role of mTOR in regulating the MDSPC dysfunction that occurs with accelerated aging. We show that mTOR signaling pathways are activated in Ercc1(-/Δ) MDSPCs compared with wild-type (WT) MDSPCs. Additionally, inhibiting mTOR with rapamycin promoted autophagy and improved the myogenic differentiation capacity of the Ercc1(-/Δ) MDSPCs. The percent of apoptotic and senescent cells in Ercc1(-/Δ) MDSPC cultures was decreased upon mTOR inhibition. These results establish that mTOR signaling contributes to stem cell dysfunction and cell fate decisions in response to endogenous DNA damage. Therefore, mTOR represents a potential therapeutic target for improving defective, aged stem cells.

Published
2017

7. Intra‐articular autologous uncultured adipose‐derived stromal cell transplantation inhibited the progression of cartilage degeneration

Author

Kuroda, Yuichi, primary, Matsumoto, Tomoyuki, additional, Hayashi, Shinya, additional, Hashimoto, Shingo, additional, Takayama, Koji, additional, Kirizuki, Shinsuke, additional, Tsubosaka, Masanori, additional, Kamenaga, Tomoyuki, additional, Takashima, Yoshinori, additional, Matsushita, Takehiko, additional, Niikura, Takahiro, additional, and Kuroda, Ryosuke, additional

Published
2018
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8. Intra‐articular autologous uncultured adipose‐derived stromal cell transplantation inhibited the progression of cartilage degeneration.

Author

Kuroda, Yuichi, Matsumoto, Tomoyuki, Hayashi, Shinya, Hashimoto, Shingo, Takayama, Koji, Kirizuki, Shinsuke, Tsubosaka, Masanori, Kamenaga, Tomoyuki, Takashima, Yoshinori, Matsushita, Takehiko, Niikura, Takahiro, and Kuroda, Ryosuke

Subjects
STROMAL cells, CELL transplantation, CARTILAGE, INTRA-articular injections, GROWTH factors, MELPHALAN
Abstract

The role of uncultured adipose‐derived stromal cells for osteoarthritis treatment remains unclear despite sporadic reports supporting their use in clinical settings. This study aimed to evaluate the therapeutic effects of autologous uncultured adipose‐derived stromal cell transplantation in a rabbit osteoarthritis model. Uncultured adipose‐derived stromal cells isolated from rabbits were administered via intra‐articular injection into the knees after osteoarthritis onset. Animals were sacrificed at 8 and 12 weeks after osteoarthritis onset to compare the macroscopic, histological, and immunohistochemical characteristics between the uncultured adipose‐derived stromal cell and control groups. Co‐culture assay was also performed. The chondrocytes isolated from the model were co‐cultured with adipose‐derived stromal cells. The cell viability of chondrocytes and expression of chondrocyte‐specific genes in the co‐culture (uncultured adipose‐derived stromal cell) group were compared with the mono‐culture (control; chondrocytes only) group. In macroscopic and histological analyses, the uncultured adipose‐derived stromal cell group showed less damage to the cartilage surface than the control group at 8 and 12 weeks after osteoarthritis onset. In immunohistochemical and co‐culture assay, the uncultured adipose‐derived stromal cell group showed higher expression of collagen type II and SRY box‐9 and lower expression of matrix metalloproteinase‐13 than the control group. The cell viability of chondrocytes in the uncultured adipose‐derived stromal cell group was higher than that in the control group. Intra‐articular autologous uncultured adipose‐derived stromal cell transplantation inhibited the progression of cartilage degeneration in a rabbit osteoarthritis model by regulating chondrocyte viability and secreting chondrocyte‐protecting cytokines or growth factors, which promote anabolic factors and inhibit catabolic factors. © 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:1376–1386, 2019. [ABSTRACT FROM AUTHOR]

Published
2019
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9. mTOR signaling plays a critical role in the defects observed in muscle-derived stem/progenitor cells isolated from a murine model of accelerated aging

Author

Takayama, Koji, primary, Kawakami, Yohei, additional, Lavasani, Mitra, additional, Mu, Xiaodong, additional, Cummins, James H., additional, Yurube, Takashi, additional, Kuroda, Ryosuke, additional, Kurosaka, Masahiro, additional, Fu, Freddie H., additional, Robbins, Paul D., additional, Niedernhofer, Laura J., additional, and Huard, Johnny, additional

Published
2016
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