1. Human papillomavirus DNA in oral squamous cell carcinomas and normal mucosa.
- Author
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Ostwald, C, Müller, P, Barten, M, Rutsatz, K, Sonnenburg, M, Milde-Langosch, K, and Löning, T
- Subjects
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COMPARATIVE studies , *DNA , *DOCUMENTATION , *IMMUNOBLOTTING , *RESEARCH methodology , *MEDICAL cooperation , *MOUTH tumors , *NUCLEIC acid probes , *NUCLEOTIDES , *NUCLEOTIDE separation , *PAPILLOMAVIRUSES , *POLYMERASE chain reaction , *RESEARCH , *SQUAMOUS cell carcinoma , *EVALUATION research - Abstract
Human papillomavirus (HPV) infections in oral carcinomas and normal oral mucosa were studied by consensus primer screening and typing for HPV types 6/11, 16 and 18 DNA. After polymerase chain reaction (PCR) the DNA species of interest were identified by Southern blot hybridization with digoxigenin-labeled oligonucleotide probes. Frozen tissue and scrapings were equally suitable for HPV testing and yielded high HPV detection rates in carcinomas. By comparison, HPV analysis of paraffin-embedded material was much less efficient. HPV were demonstrated in 61.5% (16/26) of oral squamous cell carcinomas, high risk HPV 16 and 18 being the preferential types. The frequency of HPV detection in non-neoplastic mucosa of tumor patients decreased clearly with increasing distance from the tumor (range 26.9-3.8%) suggesting focal HPV infections. In contrast, normal buccal mucosa of a group of healthy volunteers contained HPV DNA only in 1% (1/97). [ABSTRACT FROM AUTHOR]
- Published
- 1994
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