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Objective: We sought to test whether a 2-week Total Worker Health (TWH) training mapped to TWH education competencies could be administered to a Mexican audience of occupational safety and health professionals and could lead to positive changes to knowledge and behaviors., Methods: This study used robust program evaluation methods collected before and after each of the nine training days and at the end of the course., Results: Overall course quality received a mean score of 4.6 (SD = 0.6) and 98.8% of participants agreed that their TWH knowledge increased. All participants intended to make at least one change to their professional practice, most frequently helping companies assess their organizational culture to support health, safety, and well-being., Conclusions: This TWH training was well received and led to positive self-reported increase in knowledge and abilities to influence workers' health, safety, and well-being., Competing Interests: Conflict of interest: The University of Colorado developed a Memorandum of Understanding with the Instituto Mexicano del Seguro Social (IMSS), which funded the development and implementation of the Total Worker Health® training. The IMSS selected the employees who would participate in the training. The IMSS had no role in evaluation data collection, analysis, or interpretation. The corresponding author had full access to all the raw evaluation data and had final responsibility for the decision to submit for publication. No additional conflicts of interest declared from any author., (Copyright © 2024 American College of Occupational and Environmental Medicine.)